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All rights reserved." } ], "http://purl.org/dc/elements/1.1/title" : [ { "@value" : "BEL Framework Large Corpus Document" } ], "http://purl.org/pav/authoredBy" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAziS8tMMHgeWsD7Q45H6zJLqnx2TaN42PF6Y3DBYsSfs#_7" } ], "http://purl.org/pav/version" : [ { "@value" : "1.4" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAziS8tMMHgeWsD7Q45H6zJLqnx2TaN42PF6Y3DBYsSfs#_6", "http://www.w3.org/ns/prov#value" : [ { "@value" : "BACKGROUND: Previous studies have indicated that catechol-O-methyltransferase (COMT) can modulate renal dopaminergic tone. OBJECTIVE: To test the hypothesis that COMT blockade protects from salt-induced hypertension. METHODS: COMT gene-disrupted (-/-) mice and wild-type controls received a high-sodium diet (NaCl 6%) for 3 weeks. Blood pressure and heart rate were recorded by radiotelemetry. Tissue and urine samples were assessed by light microscopy and high-performance liquid chromatography. The effects of nitecapone treatment were also examined. Systolic blood pressure and heart rate during normal sodium diet were similar in COMT (-/-) and wild-type mice. The high-sodium diet increased night-time systolic and diastolic blood pressures in wild-type mice, whereas blood pressure in COMT (-/-) mice remained unaltered. In wild-type mice, the sodium-induced increase in blood pressure was completely normalized by treatment with the COMT inhibitor, nitecapone. At baseline, 24-h urinary excretion of levodopa (L-DOPA), dopamine and noradrenaline was increased by 145, 85 and 74%, respectively, in COMT (-/-) mice compared with wild-type controls. In COMT (-/-) and wild-type mice, a high-sodium diet increased urinary L-DOPA excretion by 405 and 660% (reflected as 102 and 212% increases in dopamine excretion), respectively. The absolute amounts of urinary L-DOPA and dopamine remained 60 and 20% greater in COMT (-/-) mice. The high-sodium diet did not influence renal cortical COMT activity. CONCLUSION: Our findings suggest that COMT deficiency in mice increases the availability of L-DOPA, leading to enhanced dopaminergic tone, which may be associated with resistance to salt-induced hypertension. The findings of the present study also underline the importance of COMT in the regulation of blood pressure, sodium excretion and renal dopaminergic tone." } ], "http://www.w3.org/ns/prov#wasQuotedFrom" : [ { "@id" : "http://www.ncbi.nlm.nih.gov/pubmed/14654758" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAziS8tMMHgeWsD7Q45H6zJLqnx2TaN42PF6Y3DBYsSfs#_7", "http://www.w3.org/2000/01/rdf-schema#label" : [ { "@value" : "Selventa" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAziS8tMMHgeWsD7Q45H6zJLqnx2TaN42PF6Y3DBYsSfs#assertion", "http://www.w3.org/ns/prov#hadPrimarySource" : [ { "@id" : "http://www.ncbi.nlm.nih.gov/pubmed/14654758" } ], "http://www.w3.org/ns/prov#wasDerivedFrom" : [ { "@id" : "http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel" }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAziS8tMMHgeWsD7Q45H6zJLqnx2TaN42PF6Y3DBYsSfs#_6" } ] } ], "@id" : "http://www.tkuhn.ch/bel2nanopub/RAziS8tMMHgeWsD7Q45H6zJLqnx2TaN42PF6Y3DBYsSfs#provenance" }, { "@graph" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAziS8tMMHgeWsD7Q45H6zJLqnx2TaN42PF6Y3DBYsSfs", "http://purl.org/dc/terms/created" : [ { "@type" : "http://www.w3.org/2001/XMLSchema#dateTime", "@value" : "2014-07-03T14:30:18.819+02:00" } ], "http://purl.org/pav/createdBy" : [ { "@id" : "http://orcid.org/0000-0001-6818-334X" }, { "@id" : "http://orcid.org/0000-0002-1267-0234" } ] } ], "@id" : "http://www.tkuhn.ch/bel2nanopub/RAziS8tMMHgeWsD7Q45H6zJLqnx2TaN42PF6Y3DBYsSfs#pubinfo" } ]