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All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAxvIkeqNawm4H4L5EIPWzMMcsT0J6YoEzdlphnTWucJU#_5 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAxvIkeqNawm4H4L5EIPWzMMcsT0J6YoEzdlphnTWucJU#_4 http://www.w3.org/ns/prov#value Protein kinase C (PKC) regulates cystic fibrosis transmembrane conductance regulator (CFTR) channel activity but the PKC signaling mechanism is not yet known. The goal of these studies was to identify PKC isotype(s) required for control of CFTR function. CFTR activity was measured as 36Cl efflux in a Chinese hamster ovary cell line stably expressing wild-type CFTR (CHO-wtCFTR) and in a Calu-3 cell line. Chelerythrine, a PKC inhibitor, delayed increased CFTR activity induced with phorbol 12-myristate 13-acetate or with the cAMP-generating agents (-)-epinephrine or forskolin plus 8-(4-chlorophenylthio)adenosine 3',5'- cyclic monophosphate. Immunoblot analysis of Calu-3 cells revealed that PKC-alpha, -betaII, -delta, -epsilon, and -zeta were expressed in confluent cell cultures. Pretreatment of cell monolayers with Lipofectin plus antisense oligonucleotide to PKC-epsilon for 48 h prevented stimulation of CFTR with (-)-epinephrine, reduced PKC-epsilon activity in unstimulated cells by 52.1%, and decreased PKC-epsilon mass by 76.1% but did not affect hormone-activated protein kinase A activity. Sense oligonucleotide to PKC-epsilon and antisense oligonucleotide to PKC-delta and -zeta did not alter (-)-epinephrine-stimulated CFTR activity. These results demonstrate the selective regulation of CFTR function by constitutively active PKC-epsilon. http://www.tkuhn.ch/bel2nanopub/RAxvIkeqNawm4H4L5EIPWzMMcsT0J6YoEzdlphnTWucJU#_4 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/9814985 http://www.tkuhn.ch/bel2nanopub/RAxvIkeqNawm4H4L5EIPWzMMcsT0J6YoEzdlphnTWucJU#_5 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAxvIkeqNawm4H4L5EIPWzMMcsT0J6YoEzdlphnTWucJU#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/9814985 http://www.tkuhn.ch/bel2nanopub/RAxvIkeqNawm4H4L5EIPWzMMcsT0J6YoEzdlphnTWucJU#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAxvIkeqNawm4H4L5EIPWzMMcsT0J6YoEzdlphnTWucJU#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAxvIkeqNawm4H4L5EIPWzMMcsT0J6YoEzdlphnTWucJU#_4 http://www.tkuhn.ch/bel2nanopub/RAxvIkeqNawm4H4L5EIPWzMMcsT0J6YoEzdlphnTWucJU#pubinfo http://www.tkuhn.ch/bel2nanopub/RAxvIkeqNawm4H4L5EIPWzMMcsT0J6YoEzdlphnTWucJU http://purl.org/dc/terms/created 2014-07-03T14:31:00.743+02:00 http://www.tkuhn.ch/bel2nanopub/RAxvIkeqNawm4H4L5EIPWzMMcsT0J6YoEzdlphnTWucJU http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAxvIkeqNawm4H4L5EIPWzMMcsT0J6YoEzdlphnTWucJU http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234