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All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAwt6dY5LSOb-oA3KDBPvkEREXKotP_ENPk-e0vkAg0hg#_4 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RAwt6dY5LSOb-oA3KDBPvkEREXKotP_ENPk-e0vkAg0hg#_3 http://www.w3.org/ns/prov#value The mRNA encoding the rat ATP synthase beta-subunit was rapidly induced by nerve growth factor, within 60 min, in cultured adult rat dorsal root ganglion neurons. ATP synthase beta-subunit cDNA clones were isolated from a lambda library. The library was constructed using rat dorsal root ganglion mRNA that was differentially screened with cDNA-derived probes from untreated and nerve-growth-factor-treated primary cultures of adult rat dorsal root ganglion sensory neurons. Radiolabelled probes were made from submicrogram quantities of RNA, by a novel PCR-based technique, which allows small amounts of primary tissue to be used for library screening. The use of this technique in isolating novel differentially expressed mRNAs is discussed. http://www.tkuhn.ch/bel2nanopub/RAwt6dY5LSOb-oA3KDBPvkEREXKotP_ENPk-e0vkAg0hg#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/8612603 http://www.tkuhn.ch/bel2nanopub/RAwt6dY5LSOb-oA3KDBPvkEREXKotP_ENPk-e0vkAg0hg#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAwt6dY5LSOb-oA3KDBPvkEREXKotP_ENPk-e0vkAg0hg#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/8612603 http://www.tkuhn.ch/bel2nanopub/RAwt6dY5LSOb-oA3KDBPvkEREXKotP_ENPk-e0vkAg0hg#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAwt6dY5LSOb-oA3KDBPvkEREXKotP_ENPk-e0vkAg0hg#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAwt6dY5LSOb-oA3KDBPvkEREXKotP_ENPk-e0vkAg0hg#_3 http://www.tkuhn.ch/bel2nanopub/RAwt6dY5LSOb-oA3KDBPvkEREXKotP_ENPk-e0vkAg0hg#pubinfo http://www.tkuhn.ch/bel2nanopub/RAwt6dY5LSOb-oA3KDBPvkEREXKotP_ENPk-e0vkAg0hg http://purl.org/dc/terms/created 2014-07-03T14:33:50.954+02:00 http://www.tkuhn.ch/bel2nanopub/RAwt6dY5LSOb-oA3KDBPvkEREXKotP_ENPk-e0vkAg0hg http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAwt6dY5LSOb-oA3KDBPvkEREXKotP_ENPk-e0vkAg0hg http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234