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All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAwpbp9W9kQfFl9RJ2bFfbaqRKtnkJpJTb_VHO1KghTSA#_4 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RAwpbp9W9kQfFl9RJ2bFfbaqRKtnkJpJTb_VHO1KghTSA#_3 http://www.w3.org/ns/prov#value Although L-FABP is an important binding site for various hydrophobic ligands in hepatocytes, its in vivo significance is not understood. We have therefore created L-FABP null mice and report here their initial analysis, focusing on the impact of this mutation on hepatic fatty acid binding capacity, lipid composition, and expression of other lipid binding proteins. Gel-filtered cytosol from L-FABP null liver lacked the main fatty acid binding peak in the fraction that normally comprises both L-FABP and sterol carrier protein-2 (SCP-2). The binding capacity for cis-parinaric acid was decreased >80% in this region. Molar ratios of cholesterol/cholesterol ester, cholesteryl ester/triglyceride, and cholesterol/phospholipid were 2-3 fold greater, reflecting up to 3-fold absolute increases in specific lipid classes in the order cholesterol > cholesterol esters > phospholipids. In contrast, the liver pool sizes of nonesterified fatty acids and triglycerides were not altered. However, hepatic deposition of a bolus of intravenously injected [14C]-oleate was markedly reduced, showing altered lipid pool turnover. An ~75% increase of soluble SCP-2, but little or no change of other soluble (glutathione-S-transferase, albumin) and membrane (fatty acid transport protein, CD36, aspartate aminotransferase, caveolin) fatty acid transporters was measured. These results (i) provide for the first time a quantitative assessment of the contribution of L-FABP to cytosolic fatty acid binding capacity, (ii) establish L-FABP as an important determinant of hepatic lipid composition and turnover, and (iii) suggest that SCP-2 contributes to the accumulation of cholesterol in L-FABP null liver. http://www.tkuhn.ch/bel2nanopub/RAwpbp9W9kQfFl9RJ2bFfbaqRKtnkJpJTb_VHO1KghTSA#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/12670956 http://www.tkuhn.ch/bel2nanopub/RAwpbp9W9kQfFl9RJ2bFfbaqRKtnkJpJTb_VHO1KghTSA#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAwpbp9W9kQfFl9RJ2bFfbaqRKtnkJpJTb_VHO1KghTSA#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/12670956 http://www.tkuhn.ch/bel2nanopub/RAwpbp9W9kQfFl9RJ2bFfbaqRKtnkJpJTb_VHO1KghTSA#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAwpbp9W9kQfFl9RJ2bFfbaqRKtnkJpJTb_VHO1KghTSA#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAwpbp9W9kQfFl9RJ2bFfbaqRKtnkJpJTb_VHO1KghTSA#_3 http://www.tkuhn.ch/bel2nanopub/RAwpbp9W9kQfFl9RJ2bFfbaqRKtnkJpJTb_VHO1KghTSA#pubinfo http://www.tkuhn.ch/bel2nanopub/RAwpbp9W9kQfFl9RJ2bFfbaqRKtnkJpJTb_VHO1KghTSA http://purl.org/dc/terms/created 2014-07-03T14:32:00.715+02:00 http://www.tkuhn.ch/bel2nanopub/RAwpbp9W9kQfFl9RJ2bFfbaqRKtnkJpJTb_VHO1KghTSA http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAwpbp9W9kQfFl9RJ2bFfbaqRKtnkJpJTb_VHO1KghTSA http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234