@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAumDYACtYZ2LIBMrfMJRRHp1a-DJLzLjRLCtYwXDyFsE> .
@prefix sub: <http://purl.org/np/RAumDYACtYZ2LIBMrfMJRRHp1a-DJLzLjRLCtYwXDyFsE#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:_1 rdfs:label "cells" .
  
  sub:_2 rdfs:label "TRIzol" .
  
  sub:step dce:description "At the indicated time points (0, 2, 4, 8, 12, 16, 20, 24, 28 h post-infection), cells were rinsed with 10 ml Phosphate Buffered Saline (PBS) buffer once and lysed in 1 ml TRIzol for 5 min at room temperature.";
    a pplan:Step;
    bs:bioSampleUsed sub:_1;
    bs:reagentUsed sub:_2, <http://www.bioassayontology.org/bao#BAO_0000012> .
  
  <http://www.bioassayontology.org/bao#BAO_0000012> rdfs:label "Phosphate Buffered Saline (PBS) buffer" .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-19T15:27:20.144763"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:5_4i5n3wEeuHHedpwQte0g, authority:_BhhVn3wEeue-xf0uhMXow,
      authority:rgQ_nn3wEeu0G5-yVfVEZg, authority:wc3PBH3wEeuUWht40pM97Q .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "PO094v9/qwnhWkJRhyMQ0ZeY63Vf94p6IYev3/7TbTeHFcakDXBhhHpAK3eQSP86V38o+02FVAv1UvOqfPycPzlcNBDj9ZhD+gNaR0q6OKYkKlwKXP1QJgOrPnJu3YNYHSi1EQ7oDwHmsv9+8BG+3XXy6jAHWFEmpuMLVQs8toM=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-19T15:27:20.144763"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}