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Using siRNAs targeting the mTOR complex proteins, raptor (mTORC1) and rictor (mTORC2), we depleted HCAECs of one of the two mTORCs (Fig. 1C) and measured migration toward VEGF. Knockdown of rictor, but not raptor, was effective in inhibiting HCAEC migration toward VEGF compared with HCAECs treated with nontargeting siRNA (Fig. 1D).
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