@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAtOQ8XQ7GZTwflimGKNC-I4h2G7J0zWb_1iIKupDon1c> .
@prefix sub: <http://purl.org/np/RAtOQ8XQ7GZTwflimGKNC-I4h2G7J0zWb_1iIKupDon1c#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:_1 rdfs:label "tissue sample " .
  
  sub:_2 rdfs:label "tissue sample " .
  
  sub:_3 rdfs:label "Eppendorf Safe-Lock microcentrifuge tube " .
  
  sub:_4 rdfs:label "MM300 Mixer Mill " .
  
  sub:_5 rdfs:label "glass ball" .
  
  sub:_6 rdfs:label "lysis solution." .
  
  sub:step dce:description "Eppendorf Safe-Lock microcentrifuge tube with tissue sample and glass ball (6 mm) freeze at -80°C, grind in the MM300 Mixer Mill for 2 min at 30 Hz. Alternatively, grind the sample in lysis solution.";
    sp:hasExperimentalInput sub:_1;
    a pplan:Step;
    bs:bioSampleUsed sub:_2;
    bs:labEquipmentUsed sub:_3, sub:_4, sub:_5;
    bs:reagentUsed sub:_6 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-02-14T05:41:55.504262"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:7_w36mA_Eeu0wL_Bfi0JeA, authority:DVY6KmBAEeuVkt9djdcxMQ,
      authority:JS9xMm6xEeucGA_9lE1cGg, authority:KCLYRmA_Eeu5Ga9oHJ_tWQ, authority:UL8NBmA_Eeu9AR-wQVwubA,
      authority:pVZdfmA_Eeu5O8v4stVOIw .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "ckIbWVKmEKKSLgN15o96fhu0t92aK7MPe8btTgANkeDwnHjIGbCTOlFbMmAIid8sORwr0sqtV8UPJD6YzPAUql7RCZuA2j8a4XdxNPeLYQyPnM82/+yji3tdFdDwxxjVL1kNr3kPMo+rCiCGdO2jaEEgdgONW/NNJyVnjUEni/E=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-02-14T05:41:55.504262"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}