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All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAs-dRtzKApniHB5OpSAKBDHyZ5V2iCxqX3OQayHWOrjQ#_6 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RAs-dRtzKApniHB5OpSAKBDHyZ5V2iCxqX3OQayHWOrjQ#_5 http://www.w3.org/ns/prov#value Here, we show that cotransfection with transforming growth factor (TGF)-beta-inducible early gene (TIEG)2 increased MAO B gene expression at promoter, mRNA, protein, and catalytic activity levels in both SH-SY5Y and HepG2 cells. Mutation of the CACCC element increased the MAO B promoter activity, and cotransfection with TIEG2 further increased the promoter activity, suggesting that CACCC was a repressor element. This increase was reduced when the proximal Sp1 overlapping sites was mutated. Similar interactions were found with Sp3. These results showed that TIEG2 and Sp3 were repressors at the CACCC element but were activators at proximal Sp1 overlapping sites of MAO B. Gel-shift and chromatin immunoprecipitation assays showed that TIEG2 and Sp3 bound directly to CACCC element and the proximal Sp1 sites in both synthetic oligonucleotides and natural MAO B core promoter. TIEG2 had a higher affinity to Sp1 sites than CACCC element, whereas Sp3 had an equal affinity to both elements. Thus, TIEG2 was an activator, but Sp3 had no effect on MAO B gene expression. http://www.tkuhn.ch/bel2nanopub/RAs-dRtzKApniHB5OpSAKBDHyZ5V2iCxqX3OQayHWOrjQ#_5 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/15024015 http://www.tkuhn.ch/bel2nanopub/RAs-dRtzKApniHB5OpSAKBDHyZ5V2iCxqX3OQayHWOrjQ#_6 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAs-dRtzKApniHB5OpSAKBDHyZ5V2iCxqX3OQayHWOrjQ#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/15024015 http://www.tkuhn.ch/bel2nanopub/RAs-dRtzKApniHB5OpSAKBDHyZ5V2iCxqX3OQayHWOrjQ#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAs-dRtzKApniHB5OpSAKBDHyZ5V2iCxqX3OQayHWOrjQ#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAs-dRtzKApniHB5OpSAKBDHyZ5V2iCxqX3OQayHWOrjQ#_5 http://www.tkuhn.ch/bel2nanopub/RAs-dRtzKApniHB5OpSAKBDHyZ5V2iCxqX3OQayHWOrjQ#pubinfo http://www.tkuhn.ch/bel2nanopub/RAs-dRtzKApniHB5OpSAKBDHyZ5V2iCxqX3OQayHWOrjQ http://purl.org/dc/terms/created 2014-07-03T14:32:17.909+02:00 http://www.tkuhn.ch/bel2nanopub/RAs-dRtzKApniHB5OpSAKBDHyZ5V2iCxqX3OQayHWOrjQ http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAs-dRtzKApniHB5OpSAKBDHyZ5V2iCxqX3OQayHWOrjQ http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234