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All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RArS4LThs60mxNL93czgfJDwEKJ_Wx2x7nTEVuLLnZQKI#_5 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RArS4LThs60mxNL93czgfJDwEKJ_Wx2x7nTEVuLLnZQKI#_4 http://www.w3.org/ns/prov#value An early confluent culture of C2C12 cells pretreated with 10 microg/ml of cycloheximide (CHX) for 30 minutes and then treated with 300ng/ml of BMP-2 (bone morphogenetic protein2) for 1 day induced the expression of Dlx5. Moreover when Dlx5 was over-expressed by transfecting C2C12 cells with the Dlx5 expression vector, Alp expression was stimulated even in the absence of BMP-2 treatment and that Dlx5 and the product of its target gene, Runx2, stimulated ALP promoter activity in an additive manner. However, because Dlx5 continued to stimulate ALP expression in Runx2(-/-) cells, the ALP stimulatory activity of Dlx5 is independent of Runx2. Thus it suggests that Alp is a downstream target of Dlx5 and that Dlx5 and Runx5 can both regulate its expression. http://www.tkuhn.ch/bel2nanopub/RArS4LThs60mxNL93czgfJDwEKJ_Wx2x7nTEVuLLnZQKI#_4 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/15383550 http://www.tkuhn.ch/bel2nanopub/RArS4LThs60mxNL93czgfJDwEKJ_Wx2x7nTEVuLLnZQKI#_5 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RArS4LThs60mxNL93czgfJDwEKJ_Wx2x7nTEVuLLnZQKI#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/15383550 http://www.tkuhn.ch/bel2nanopub/RArS4LThs60mxNL93czgfJDwEKJ_Wx2x7nTEVuLLnZQKI#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RArS4LThs60mxNL93czgfJDwEKJ_Wx2x7nTEVuLLnZQKI#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RArS4LThs60mxNL93czgfJDwEKJ_Wx2x7nTEVuLLnZQKI#_4 http://www.tkuhn.ch/bel2nanopub/RArS4LThs60mxNL93czgfJDwEKJ_Wx2x7nTEVuLLnZQKI#pubinfo http://www.tkuhn.ch/bel2nanopub/RArS4LThs60mxNL93czgfJDwEKJ_Wx2x7nTEVuLLnZQKI http://purl.org/dc/terms/created 2014-07-03T14:32:32.101+02:00 http://www.tkuhn.ch/bel2nanopub/RArS4LThs60mxNL93czgfJDwEKJ_Wx2x7nTEVuLLnZQKI http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RArS4LThs60mxNL93czgfJDwEKJ_Wx2x7nTEVuLLnZQKI http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234