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@prefix schema: <http://schema.org/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
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@prefix bibo: <http://purl.org/ontology/bibo/> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix foaf: <http://xmlns.com/foaf/0.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubinfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.org/coloc-seq/mock-coloc-experiment> schema:description "Control experiment with detergent-permeabilized membranes to determine baseline RNase protection and validate method specificity"@en;
    schema:methodology "Treatment with detergents (Triton X-100, digitonin) to solubilize membrane compartments while maintaining protein structures"@en;
    schema:name "Mock CoLoC-seq"@en;
    schema:purpose "Establish P0=0 baseline (no genuine organellar protection in dissolved membranes)"@en;
    a schema:Thing;
    rdfs:comment "Critical validation that observed protection genuinely reflects organellar localization and not artifact of RNA secondary structure or RNase resistance"@en .
  
  this: schema:about <https://doi.org/10.1093/nar/gkac1205>;
    schema:text "The mock CoLoC-seq control experiment uses detergent-permeabilized membranes to detect false positives and distinguish genuine organellar protection from intrinsic RNase resistance."@en;
    a <https://w3id.org/np/RAqWgWTy_lLfUrvdgqP0lggMHH_Of_5TgmpDWkpxe16gg/o/ntemplate/AIDA-Sentence>;
    rdfs:seeAlso <http://purl.org/coloc-seq/method> .
}

sub:provenance {
  <https://doi.org/10.1093/nar/gkac1205> bibo:doi "10.1093/nar/gkac1205";
    schema:datePublished "2023"^^xsd:gYear;
    schema:isPartOf sub:__1;
    schema:name "CoLoC-seq probes the global topology of organelle transcriptomes"@en;
    a bibo:AcademicArticle, schema:ScholarlyArticle .
  
  sub:__1 schema:issn "0305-1048";
    schema:name "Nucleic Acids Research"@en;
    a schema:Periodical .
  
  sub:assertion prov:wasAttributedTo orcid:0000-0001-8763-6703, orcid:0000-0002-1481-2996,
      orcid:0000-0002-9142-7979;
    prov:wasDerivedFrom <https://doi.org/10.1093/nar/gkac1205> .
}

sub:pubinfo {
  orcid:0009-0005-7752-8203 foaf:name "Tiado Kollender" .
  
  this: dct:created "2026-01-11T00:00:00Z"^^xsd:dateTime;
    dct:license <https://creativecommons.org/publicdomain/zero/1.0/>;
    npx:signedBy orcid:0009-0005-7752-8203;
    npx:wasCreatedFromPubinfoTemplate <https://w3id.org/np/RA0J4vUn_dekg-U1kK3AOEt02p9mT2WO03uGxLDec1jLw>,
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    pav:createdBy orcid:0009-0005-7752-8203;
    a npx:ExampleNanopub;
    rdfs:comment "AIDA-based assertion describing the mock control experiment validation strategy"@en;
    rdfs:label "Nanopublication: CoLoC-seq mock control experiment design"@en;
    prov:wasAttributedTo orcid:0009-0005-7752-8203 .
  
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