. . . . "Control experiment with detergent-permeabilized membranes to determine baseline RNase protection and validate method specificity"@en . "Treatment with detergents (Triton X-100, digitonin) to solubilize membrane compartments while maintaining protein structures"@en . "Mock CoLoC-seq"@en . "Establish P0=0 baseline (no genuine organellar protection in dissolved membranes)"@en . . "Critical validation that observed protection genuinely reflects organellar localization and not artifact of RNA secondary structure or RNase resistance"@en . . "The mock CoLoC-seq control experiment uses detergent-permeabilized membranes to detect false positives and distinguish genuine organellar protection from intrinsic RNase resistance."@en . . . "10.1093/nar/gkac1205" . "2023"^^ . . "CoLoC-seq probes the global topology of organelle transcriptomes"@en . . . "0305-1048" . "Nucleic Acids Research"@en . . . . . . "Tiado Kollender" . "2026-01-11T00:00:00Z"^^ . . . . . . . "AIDA-based assertion describing the mock control experiment validation strategy"@en . "Nanopublication: CoLoC-seq mock control experiment design"@en . . "RSA" . "MIIBIjANBgkqhkiG9w0BAQEFAAOCAQ8AMIIBCgKCAQEAoSR/rqhnkTPQO8u4l2JQAemJo5cN83rT54nA5NXHUqaeu26bEjT/rsX1PCVBhhJ3cV2uu4wjI0NfcVaKPKmODSf6p579tdOykOnjbWLbxxPMe5DC5eV1ThMHsFsNU/6vCYIfS00E7wre7fnFYz7FAG7v1MZEyWeSXsHgk9jHoqQQDPqup5PHReKp1dd6J0Nht/OfSYnUancKAgIFyPhkTlThsLK5xSNyNEgiTR7pwkP5pGq2AnD6bPw4G3kiPnIpIGZNsaeIOvhn64gwbrtoJVdoun/namw6FQ7StEC5km/0evaBZc4qIextwO/s32Wru77ukblJ0Xi/YzbC0iLuIQIDAQAB" . "h943vqtTQSG4EhmGImSqiHeuu7qOmPwkoGprStrJ8S3k5vdDtygZ4QDi27/pi+m0v/EyXNNV/E58FGAhGXe+M72goss27AtCEZyruArgheV9mxkfZG0XVSGsCRkkUFM4uGOo97M9e0fg2k8Wml/od4VKO0avSOssMzb4EQujXgz77XEicX5zLTRtqRozFpXzr0SureAeksIyztH4J6DVUpxBXfoychCEl/7XJXbFLdfUXSzBMz5Asdvsjp9hlkWHK23QcrOtJ8BXOUDpkiEseMaCQKrYynw2+dIHX7v9KZCDfStea3iShrpEEY2oDBV2UBzvS2d18UEwYXSXAnZjaA==" . .