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All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RApyTC93SSjIHwzrjCaSolcRpkfIN5M4GcPRH9LYt1IXU#_4 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RApyTC93SSjIHwzrjCaSolcRpkfIN5M4GcPRH9LYt1IXU#_3 http://www.w3.org/ns/prov#value The differential expression of the three genes was confirmed by RT-PCR using their respective gene-specific primers. cAMP-dependent phosphodiesterase shows about 4.5-fold increase in RNA from dbcAMP-treated astrocytes using a template amount of 10 ng, confirming the differential expression (Fig. 2). For BAALC, about 1.9-fold increase was seen in RNA from dbcAMP-treated astrocytes as compared to the control RNA (Fig. 3A) using a template amount of 20 ng. The expression of BAALC was also analyzed by RT-PCR in C6 glioma cells (Fig. 3B) treated with the differentiation inducing agents, viz. dbcAMP (1 mM), and staurosporine (1 nM). BAALC was found to be up regulated in dbc-treated C6 cells (9.4-fold) and to less extent in staurosporine-treated cells (1.7-fold). Up regulation of BAALC expression is thus observed both in normal and malignant astrocytes on differentiation. RT-PCR analysis demonstrated that RAMP3 expression is about 6.3-fold in control (DMSO) treated astrocytes as compared to those treated with staurosporine with a template amount of 10 ng (Fig. 4). http://www.tkuhn.ch/bel2nanopub/RApyTC93SSjIHwzrjCaSolcRpkfIN5M4GcPRH9LYt1IXU#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/16376586 http://www.tkuhn.ch/bel2nanopub/RApyTC93SSjIHwzrjCaSolcRpkfIN5M4GcPRH9LYt1IXU#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RApyTC93SSjIHwzrjCaSolcRpkfIN5M4GcPRH9LYt1IXU#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/16376586 http://www.tkuhn.ch/bel2nanopub/RApyTC93SSjIHwzrjCaSolcRpkfIN5M4GcPRH9LYt1IXU#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RApyTC93SSjIHwzrjCaSolcRpkfIN5M4GcPRH9LYt1IXU#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RApyTC93SSjIHwzrjCaSolcRpkfIN5M4GcPRH9LYt1IXU#_3 http://www.tkuhn.ch/bel2nanopub/RApyTC93SSjIHwzrjCaSolcRpkfIN5M4GcPRH9LYt1IXU#pubinfo http://www.tkuhn.ch/bel2nanopub/RApyTC93SSjIHwzrjCaSolcRpkfIN5M4GcPRH9LYt1IXU http://purl.org/dc/terms/created 2014-07-03T14:32:54.287+02:00 http://www.tkuhn.ch/bel2nanopub/RApyTC93SSjIHwzrjCaSolcRpkfIN5M4GcPRH9LYt1IXU http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RApyTC93SSjIHwzrjCaSolcRpkfIN5M4GcPRH9LYt1IXU http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234