. . . . "Mathematical model describing RNA degradation dynamics using enzymatic kinetics"@en . "f(A) = (1 - P0) * exp(-k'i * A) + P0"@en . "CoLoC-seq Kinetics Model"@en . "P0"@en . "k'i"@en . . "Model assumes fixed time t=10 min; R0 = initial RNA concentration; A = RNase A concentration"@en . "Proportion of RNA protected from RNase digestion by organellar membranes"@en . "0 < P0 < 1: partially protected"@en . "P0 = 0: entirely accessible contaminant"@en . "P0 ≈ 1: fully protected resident RNA"@en . "Protected Pool Parameter (P0)"@en . "0 to 1 (0% to 100%)"@en . . "Primary parameter of interest for CoLoC-seq; determined by nonlinear curve fitting"@en . "Pseudo-first-order rate constant for RNA degradation by RNase"@en . "Reflects sensitivity of each transcript to RNase degradation"@en . "Digestion Rate Constant (k'i)"@en . "per μg/ml RNase A"@en . . "Fitted using Leverberg Marquardt regression; higher k' indicates faster degradation"@en . . . "The CoLoC-seq kinetics model uses the protected pool parameter P0 to distinguish genuinely localised organellar transcripts from surface-attached contaminants based on their degradation dynamics."@en . . "10.1093/nar/gkac1205" . "2023"^^ . . "CoLoC-seq probes the global topology of organelle transcriptomes"@en . . "Nucleic Acids Research"@en . . . . . "Tiado Kollender" . "2026-01-11T00:00:00Z"^^ . . . . . . "AIDA-based assertion about the CoLoC-seq kinetics model and the P0 parameter"@en . "Nanopublication: CoLoC-seq kinetics model and protected pool parameter"@en . . "RSA" . "MIIBIjANBgkqhkiG9w0BAQEFAAOCAQ8AMIIBCgKCAQEAoSR/rqhnkTPQO8u4l2JQAemJo5cN83rT54nA5NXHUqaeu26bEjT/rsX1PCVBhhJ3cV2uu4wjI0NfcVaKPKmODSf6p579tdOykOnjbWLbxxPMe5DC5eV1ThMHsFsNU/6vCYIfS00E7wre7fnFYz7FAG7v1MZEyWeSXsHgk9jHoqQQDPqup5PHReKp1dd6J0Nht/OfSYnUancKAgIFyPhkTlThsLK5xSNyNEgiTR7pwkP5pGq2AnD6bPw4G3kiPnIpIGZNsaeIOvhn64gwbrtoJVdoun/namw6FQ7StEC5km/0evaBZc4qIextwO/s32Wru77ukblJ0Xi/YzbC0iLuIQIDAQAB" . "B4f0k2iJ6+ikAZFa22wAWSHXJH+AM3Yv0srrefwQJGaI3QppiIIqhZpfx0wdYz9R8yuZOb2A6fWagOK+klQjqcoFYZ7lhXPISq0YE75KOaUH87ys4pP7XOZp/rYkliAAaLNViaft/gk6YTX2CPsZUcT+hteEwcXtqwdBH3MPFCUNn642YuGRAmhR4bAEfYwURW0lcUv94+viSDTUNMrtnjcpWwX6plei6nrTnyCaiGzIISOlIOWOOJNcGCi7xPdKFaot01+SM+3crw9aeaU3Db9SJBO+tASHfs5TLaam6OeWkrQHmlvJWGqUc2C1z3GqbAjGhdAdM5ObsuAHG0J91w==" . .