sub:assertion {
  <http://purl.org/coloc-seq/kinetics-model> schema:description "Mathematical model describing RNA degradation dynamics using enzymatic kinetics"@en ;
    schema:formula "f(A) = (1 - P0) * exp(-k'i * A) + P0"@en ;
    schema:name "CoLoC-seq Kinetics Model"@en ;
    sio:SIO_000211 "P0"@en , "k'i"@en ;
    a schema:Thing ;
    rdfs:comment "Model assumes fixed time t=10 min; R0 = initial RNA concentration; A = RNase A concentration"@en .
  <http://purl.org/coloc-seq/parameter-P0> schema:description "Proportion of RNA protected from RNase digestion by organellar membranes"@en ;
    schema:interpretation "0 < P0 < 1: partially protected"@en , "P0 = 0: entirely accessible contaminant"@en , "P0 ≈ 1: fully protected resident RNA"@en ;
    schema:name "Protected Pool Parameter (P0)"@en ;
    schema:range "0 to 1 (0% to 100%)"@en ;
    a schema:Thing ;
    rdfs:comment "Primary parameter of interest for CoLoC-seq; determined by nonlinear curve fitting"@en .
  <http://purl.org/coloc-seq/parameter-k-prime> schema:description "Pseudo-first-order rate constant for RNA degradation by RNase"@en ;
    schema:interpretation "Reflects sensitivity of each transcript to RNase degradation"@en ;
    schema:name "Digestion Rate Constant (k'i)"@en ;
    schema:unit "per μg/ml RNase A"@en ;
    a schema:Thing ;
    rdfs:comment "Fitted using Leverberg Marquardt regression; higher k' indicates faster degradation"@en .
  this: npx:relatesTo <https://w3id.org/np/RA_C-L7pLBKSyHDhS2YIFmqfb_-BLGOldcUb8emWvRltA> ;
    schema:about <https://doi.org/10.1093/nar/gkac1205> ;
    schema:text "The CoLoC-seq kinetics model uses the protected pool parameter P0 to distinguish genuinely localised organellar transcripts from surface-attached contaminants based on their degradation dynamics."@en ;
    a <https://w3id.org/np/RAnGxpTDHemPIuRP09a8Qq55oseUT_NnzlkZdSraEM0qY/o/ntemplate/AIDA-Sentence> .
}