@prefix this: <http://purl.org/np/RAn6lFSdCPdHkUH8lwoyfN6hKLdyGViZZHlimdNFP9svM> .
@prefix sub: <http://purl.org/np/RAn6lFSdCPdHkUH8lwoyfN6hKLdyGViZZHlimdNFP9svM#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sp:PhenolChloroformIsoamylAlcohol rdfs:label "500 μl phenol:chloroform:isoamyl alcohol (25:24:1) " .
  
  sub:step dc:description "Add 500 μl phenol:chloroform:isoamyl alcohol (25:24:1) to the tube. Shake vigorously to mix thoroughly. Centrifuge the samples at 12,000 x g for 15 min.";
    a p-plan:Step;
    bs:labEquipmentUsed <http://www.bioassayontology.org/bao#BAO_0010014>;
    bs:reagentUsed sp:PhenolChloroformIsoamylAlcohol .
  
  <http://www.bioassayontology.org/bao#BAO_0010014> rdfs:label "tube" .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2022-01-11T06:17:07.203997"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:9nBM7nLPEeyDqkMySNOqjw, authority:lYRLsHLPEey30Ff-lKG63A,
      authority:xM-SRHLPEey7zqurrqOmJA .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "CTcrHHbkps0g9MDJqH9QHl1OYuBn6ilru8ZCgSs0PXUi89Hj63QQoF9pLluV8zkNetVbnKs5z/jNAl9yOhdzwDnADNhx+Nk6EEH370RRnUtZOVVIWKsqMJWIjjfxw4f7kwqJUIZbwU/Xh6Q78CJ8CEKn5S5BOemmBRtgASwlJl8=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2022-01-11T06:17:07.203997"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}