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All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAm4Uqel1qiTapw4tX4GOYjOFacLmuf75lW3w9mWf9RAU#_6 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RAm4Uqel1qiTapw4tX4GOYjOFacLmuf75lW3w9mWf9RAU#_5 http://www.w3.org/ns/prov#value Overlapping putative promoter regions of human PGF gene encompassing 2-1.5 kb were cloned into reporter vectors and co-transfected into trophoblast and nontrophoblast cell lines. Promoter activity generated by a 2-1.5-kb clone was significantly higher in trophoblasts than in nontrophoblasts. Selective deletion mutants showed that a clone encompassing the PGF (2-828/++34) region generated promoter activity similar to the 2-1.5-kb region in the trophoblast. However, deletion of another 131 bp from this subclone (2-698/++34) resulted in significantly less promoter activity in the trophoblast. The (2-828/2-698) region significantly enhanced activity of a minimal promoter construct in trophoblast but not in nontrophoblast cells, suggesting that this region contributes to regulating PGF transcription in the trophoblast. Site-directed mutagenesis of a glial cell missing 1 (GCM1) motif in the 131-bp region significantly decreased enhancer activity in the trophoblast. Furthermore, overexpression of GCM1 significantly increased PGF 2-1.5-kb promoter activity and PGF mRNA expression in trophoblast and nontrophoblast cells. http://www.tkuhn.ch/bel2nanopub/RAm4Uqel1qiTapw4tX4GOYjOFacLmuf75lW3w9mWf9RAU#_5 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/18160678 http://www.tkuhn.ch/bel2nanopub/RAm4Uqel1qiTapw4tX4GOYjOFacLmuf75lW3w9mWf9RAU#_6 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAm4Uqel1qiTapw4tX4GOYjOFacLmuf75lW3w9mWf9RAU#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/18160678 http://www.tkuhn.ch/bel2nanopub/RAm4Uqel1qiTapw4tX4GOYjOFacLmuf75lW3w9mWf9RAU#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAm4Uqel1qiTapw4tX4GOYjOFacLmuf75lW3w9mWf9RAU#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAm4Uqel1qiTapw4tX4GOYjOFacLmuf75lW3w9mWf9RAU#_5 http://www.tkuhn.ch/bel2nanopub/RAm4Uqel1qiTapw4tX4GOYjOFacLmuf75lW3w9mWf9RAU#pubinfo http://www.tkuhn.ch/bel2nanopub/RAm4Uqel1qiTapw4tX4GOYjOFacLmuf75lW3w9mWf9RAU http://purl.org/dc/terms/created 2014-07-03T14:33:14.261+02:00 http://www.tkuhn.ch/bel2nanopub/RAm4Uqel1qiTapw4tX4GOYjOFacLmuf75lW3w9mWf9RAU http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAm4Uqel1qiTapw4tX4GOYjOFacLmuf75lW3w9mWf9RAU http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234