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Approximately 61,000 statements.
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Elevated PLD activity, under most circumstances, does not lead to increased phosphorylation of Akt. Increased expression of PLD1 led to increased S6 kinase phosphorylation but did not increase Akt phosphorylation in rat fibroblasts (16). However, PLD1-induced increases in S6 kinase phosphorylation were blocked by the PI3K inhibitor LY294002 (16). Similarly, LY294002 suppressed S6 kinase phosphorylation in MDA-MB-231 cells, where there is elevated expression of PLD1 and elevated PLD activity (15). However, Tee et al. (9) were able to activate mTOR with exogenously supplied PA in the presence of the PI3K inhibitor wortmannin. PLD1-induced increases in S6 kinase phosphorylation were blocked by the PI3K inhibitor LY294002
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