http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk#Head http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk#assertion http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk#provenance http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk#pubinfo http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk#assertion http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk#_1 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606 http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://amigo.geneontology.org/amigo/term/GO:0000086 http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/decreases http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://amigo.geneontology.org/amigo/term/GO:0034984 http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk#assertion http://www.w3.org/2000/01/rdf-schema#label bp(GOBP:"cellular response to DNA damage stimulus") -| bp(GOBP:"G2/M transition of mitotic cell cycle") http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk#provenance http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk#_3 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk#_2 http://www.w3.org/ns/prov#value Previous studies indicate that Kruppel-like factor 4 (KLF4 or GKLF) controls the G1/S cell cycle checkpoint upon DNA damage. We present evidence for an equally important role of KLF4 in maintaining the integrity of the G2/M checkpoint following DNA damage. HCT116, a colon cancer cell line with wild type p53 alleles, underwent sustained G2 arrest up to 4 days after gamma-irradiation. In contrast, HCT116 cells null for p53 were able to enter mitosis following irradiation. Western blot analyses of irradiated HCT116 cells showed increased levels of p53, KLF4, and p21WAF1/CIP1 and decreased levels of cyclin B1 when compared with unirradiated controls. In contrast, the levels of cyclin B1 increased in irradiated HCT116 p53-/- cells, in which KLF4 failed to increase due to the absence of p53. When KLF4 was inhibited by small interfering RNA, irradiated HCT116 cells exhibited increased mitotic indices and a rise in cyclin B1 levels. Conversely, irradiated HCT116 p53-/- cells that were infected with KLF4-expressing adenoviruses demonstrated a concurrent reduction in mitotic indices and cyclin B1 levels. In each case, Cdc2 kinase measurements showed an inverse correlation between Cdc2 kinase activities and KLF4 levels. Co-transfection experiments showed that KLF4 repressed the cyclin B1 promoter through a specific GC-rich element. Moreover, chromatin immunoprecipitation experiments demonstrated that both KLF4 and HDAC were associated with the cyclin B1 promoter in irradiated HCT116 cells. We conclude that KLF4 is essential in preventing mitotic entry following gamma-irradiation and does so by inhibiting cyclin B1 expression. http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk#_2 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/14627709 http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk#_3 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/14627709 http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk#_2 http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk#pubinfo http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk http://purl.org/dc/terms/created 2014-07-03T14:32:12.223+02:00 http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAijlzpTy3fpYD4z6sjUbvXmhKoicfKp41i8OXYuFR_Jk http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234