[ { "@graph" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAiV1cUgABMjOpzLfsNlOODF7FlMXDzV4XZLwqKH7At9k", "@type" : [ "http://www.nanopub.org/nschema#Nanopublication" ], "http://www.nanopub.org/nschema#hasAssertion" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAiV1cUgABMjOpzLfsNlOODF7FlMXDzV4XZLwqKH7At9k#assertion" } ], "http://www.nanopub.org/nschema#hasProvenance" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAiV1cUgABMjOpzLfsNlOODF7FlMXDzV4XZLwqKH7At9k#provenance" } ], "http://www.nanopub.org/nschema#hasPublicationInfo" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAiV1cUgABMjOpzLfsNlOODF7FlMXDzV4XZLwqKH7At9k#pubinfo" } ] } ], "@id" : "http://www.tkuhn.ch/bel2nanopub/RAiV1cUgABMjOpzLfsNlOODF7FlMXDzV4XZLwqKH7At9k#Head" }, { "@graph" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAiV1cUgABMjOpzLfsNlOODF7FlMXDzV4XZLwqKH7At9k#_1", "@type" : [ "http://amigo.geneontology.org/amigo/term/GO:0042789" ], "http://semanticscience.org/resource/SIO_000139" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAiV1cUgABMjOpzLfsNlOODF7FlMXDzV4XZLwqKH7At9k#_2" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAiV1cUgABMjOpzLfsNlOODF7FlMXDzV4XZLwqKH7At9k#_2", "@type" : [ "http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_36080" ], "http://purl.obolibrary.org/obo/RO_0002204" : [ { "@id" : "http://resource.belframework.org/belframework/20131211/namespace/selventa-protein-families/MEF2%20Family" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAiV1cUgABMjOpzLfsNlOODF7FlMXDzV4XZLwqKH7At9k#_3", "@type" : [ "http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement" ], "http://purl.obolibrary.org/obo/BFO_0000066" : [ { "@id" : "http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10090" } ], "http://www.w3.org/1999/02/22-rdf-syntax-ns#object" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAiV1cUgABMjOpzLfsNlOODF7FlMXDzV4XZLwqKH7At9k#_1" } ], "http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate" : [ { "@id" : "http://www.selventa.com/vocabulary/decreases" } ], "http://www.w3.org/1999/02/22-rdf-syntax-ns#subject" : [ { "@id" : "http://amigo.geneontology.org/amigo/term/GO:0007049" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAiV1cUgABMjOpzLfsNlOODF7FlMXDzV4XZLwqKH7At9k#assertion", "http://www.w3.org/2000/01/rdf-schema#label" : [ { "@value" : "bp(GOBP:\"cell cycle\") -| tscript(p(SFAM:\"MEF2 Family\"))" } ] } ], "@id" : "http://www.tkuhn.ch/bel2nanopub/RAiV1cUgABMjOpzLfsNlOODF7FlMXDzV4XZLwqKH7At9k#assertion" }, { "@graph" : [ { "@id" : "http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel", "http://purl.org/dc/elements/1.1/description" : [ { "@value" : "Approximately 61,000 statements." } ], "http://purl.org/dc/elements/1.1/rights" : [ { "@value" : "Copyright (c) 2011-2012, Selventa. All rights reserved." } ], "http://purl.org/dc/elements/1.1/title" : [ { "@value" : "BEL Framework Large Corpus Document" } ], "http://purl.org/pav/authoredBy" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAiV1cUgABMjOpzLfsNlOODF7FlMXDzV4XZLwqKH7At9k#_5" } ], "http://purl.org/pav/version" : [ { "@value" : "20131211" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAiV1cUgABMjOpzLfsNlOODF7FlMXDzV4XZLwqKH7At9k#_4", "http://www.w3.org/ns/prov#value" : [ { "@value" : "even though this fusion protein efficiently prevented BrdU uptake in MCK-CAT-positive cells (Figure 4h-j). As E2F1-pRb(SP) retained the anti-proliferative property of pRb but did not fully activate myogenesis on its own, we tested whether it could cooperate with MEF2C-VP16 in our myogenesis assays. The combination of MyoD+E12, MEF2C-VP16, and E2F1-pRb(SP) activated MCK-luciferase expression to a level equal to or greater than that achieved following co-transfection of MyoD+E12 with pRb (Figure 4a, compare columns 3 and 7), and led to the appearance of numerous cells in the culture that displayed very high levels of CAT staining and no BrdU incorporation (Figure 4k-m), very similar to what was observed following transfection of MyoD with pRb (Figure 4b-d). Cotransfection of E2F1-pRb(SP) and MEF2C-VP16 in the absence of MyoD did not induce the expression of MCK-CAT (data not shown). Thus, we were able to fully substitute for the loss of pRb by cotransfecting MyoD with an activated form of MEF2 and the cell-cycle-arresting molecule E2F1-pRb(SP)." } ], "http://www.w3.org/ns/prov#wasQuotedFrom" : [ { "@id" : "http://www.ncbi.nlm.nih.gov/pubmed/10322110" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAiV1cUgABMjOpzLfsNlOODF7FlMXDzV4XZLwqKH7At9k#_5", "http://www.w3.org/2000/01/rdf-schema#label" : [ { "@value" : "Selventa" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAiV1cUgABMjOpzLfsNlOODF7FlMXDzV4XZLwqKH7At9k#assertion", "http://www.w3.org/ns/prov#hadPrimarySource" : [ { "@id" : "http://www.ncbi.nlm.nih.gov/pubmed/10322110" } ], "http://www.w3.org/ns/prov#wasDerivedFrom" : [ { "@id" : "http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel" }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAiV1cUgABMjOpzLfsNlOODF7FlMXDzV4XZLwqKH7At9k#_4" } ] } ], "@id" : "http://www.tkuhn.ch/bel2nanopub/RAiV1cUgABMjOpzLfsNlOODF7FlMXDzV4XZLwqKH7At9k#provenance" }, { "@graph" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAiV1cUgABMjOpzLfsNlOODF7FlMXDzV4XZLwqKH7At9k", "http://purl.org/dc/terms/created" : [ { "@type" : "http://www.w3.org/2001/XMLSchema#dateTime", "@value" : "2014-07-03T14:31:30.417+02:00" } ], "http://purl.org/pav/createdBy" : [ { "@id" : "http://orcid.org/0000-0001-6818-334X" }, { "@id" : "http://orcid.org/0000-0002-1267-0234" } ] } ], "@id" : "http://www.tkuhn.ch/bel2nanopub/RAiV1cUgABMjOpzLfsNlOODF7FlMXDzV4XZLwqKH7At9k#pubinfo" } ]