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All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAhnpcR0pZ7HJ50BptPlaWVs_5cLKCuNG7rlotIrTfOG4#_4 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RAhnpcR0pZ7HJ50BptPlaWVs_5cLKCuNG7rlotIrTfOG4#_3 http://www.w3.org/ns/prov#value Finally, we reduced follistatin in skeletal myoblasts by infecting them with a retrovirus expressing a short hairpin (sh) RNA-targeting follistatin. Cells transduced with sh RNA follistatin and exposed to TSA had decreased levels of follistatin upon TSA stimulation (Figure 7D) and were less responsive to TSA compared to control cells (Figures 7E - 7G). Taken together, the results reported in this paragraph strongly suggest that myoblasts with reduced levels of follistatin become refractory to TSA treatment. FOLLISTATIN EXPRESSION IS INDUCED UPON MUSCLE INJURY AND COINCIDES WITH EXPRESSION OF MARKERS OF SATELLITE CELL ACTIVATION HDAC inhibitors induce follistatin gene expression in mouse primary myoblasts (Figure 3). To evaluate whether satellite cell activation is associated with increased follistatin gene expression, we analyzed temporal expression profiling data generated in a mouse model of muscle injury and regeneration (Zhao et al., 2002). Twelve hours after injury, the levels of follistatin were increased 7-fold and remained elevated (approximately 2-fold) for 5 days (Supplemental Figure S1). http://www.tkuhn.ch/bel2nanopub/RAhnpcR0pZ7HJ50BptPlaWVs_5cLKCuNG7rlotIrTfOG4#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/15130492 http://www.tkuhn.ch/bel2nanopub/RAhnpcR0pZ7HJ50BptPlaWVs_5cLKCuNG7rlotIrTfOG4#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAhnpcR0pZ7HJ50BptPlaWVs_5cLKCuNG7rlotIrTfOG4#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/15130492 http://www.tkuhn.ch/bel2nanopub/RAhnpcR0pZ7HJ50BptPlaWVs_5cLKCuNG7rlotIrTfOG4#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAhnpcR0pZ7HJ50BptPlaWVs_5cLKCuNG7rlotIrTfOG4#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAhnpcR0pZ7HJ50BptPlaWVs_5cLKCuNG7rlotIrTfOG4#_3 http://www.tkuhn.ch/bel2nanopub/RAhnpcR0pZ7HJ50BptPlaWVs_5cLKCuNG7rlotIrTfOG4#pubinfo http://www.tkuhn.ch/bel2nanopub/RAhnpcR0pZ7HJ50BptPlaWVs_5cLKCuNG7rlotIrTfOG4 http://purl.org/dc/terms/created 2014-07-03T14:32:20.940+02:00 http://www.tkuhn.ch/bel2nanopub/RAhnpcR0pZ7HJ50BptPlaWVs_5cLKCuNG7rlotIrTfOG4 http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAhnpcR0pZ7HJ50BptPlaWVs_5cLKCuNG7rlotIrTfOG4 http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234