sub:provenance { beldoc:dce:description "Approximately 61,000 statements." ; dce:rights "Copyright (c) 2011-2012, Selventa. All rights reserved." ; dce:title "BEL Framework Large Corpus Document" ; pav:authoredBysub:_6 ; pav:version "20131211" . sub:_5prov:value "To confirm that TNFalpha protein synthesized in the brain of the transgenic animal is biologically active, we quantified expression of TRADD protein. Homogenates of brain sampled from TNFalpha-Tg and non-Tg rats were incubated with a primary antibody that recognizes rat TRADD only after it has been activated through binding of TNFalpha to p55/TNFR1, thereby implying biological activity of the cytokine. Regional expression of TRADD protein was uniformly higher in TNFalpha-Tg rat brain than in non-Tg controls by statistically significant margins." ; prov:wasQuotedFrompubmed:18947406 . sub:_6rdfs:label "Selventa" . sub:assertionprov:hadPrimarySourcepubmed:18947406 ; prov:wasDerivedFrombeldoc: , sub:_5 . }