@prefix obo: <http://purl.obolibrary.org/obo/> .
@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ> .
@prefix sub: <http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218> rdfs:label "70% ethanol" .
  
  obo:CHEBI_16991 rdfs:label "DNA" .
  
  sub:_1 rdfs:label "pellet" .
  
  sub:_2 rdfs:label "tubes" .
  
  sub:_3 rdfs:label "TE" .
  
  sub:step dce:description "After incubation, the tubes were centrifuged at 5000 × g for 10 min at 4°C and the supernatant was gently removed. The pellet is washed two times with 1ml of 70% ethanol and the DNA is pellet by 5000 × g at 4°C for only 5 min. The supernatant is discarded and the pellet is air-dried (10 min). The pellet are allowed to re-suspend in 50 μL of TE (10 mM Tris. HCl pH 8.0; 1 mM EDTA pH 8.0).";
    sp:hasExperimentalOutput obo:CHEBI_16991;
    a pplan:Step;
    bs:bioSampleUsed obo:CHEBI_16991, sub:_1;
    bs:labEquipmentUsed sub:_2;
    bs:reagentUsed <http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218>, sub:_3 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-11T16:39:49.461208"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom <https://hypothes.is/a/-I88TIKxEeu85fuKGtcBIw>,
      authority:4n20poKxEeu9i_eN_Ca7Dw, authority:DkWQSoKyEeuQBW-OJG3rAQ, authority:KTUEYoKyEeuz26fBERP8ow,
      authority:u6u4PIKxEeuEqgdWCyMzjQ, authority:zsdcWoKxEeun64_lb_lpTg .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "WSksEo+95kLDcq4ezOI8iCMgFiMU4KLiLWhZquNxfHGJDW5UHUogYpmP0TczC4QUlpJC92X4k0iD2qF6sn6bETrWDeLagvjsZgE8MGESraGLGkK8pAoSnewc5icmi28O2jXUqNMjBCNRa1RmQmQjSVqXQFM/Im6mZWaf8gZIdGM=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-11T16:39:49.461208"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}