@prefix this: <http://www.tkuhn.ch/bel2nanopub/RAdbdqqhmT70oxtPpUr-bbOzgcSxlgqcmluHHV1q1iHKs> .
@prefix sub: <http://www.tkuhn.ch/bel2nanopub/RAdbdqqhmT70oxtPpUr-bbOzgcSxlgqcmluHHV1q1iHKs#> .
@prefix beldoc: <http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix dct: <http://purl.org/dc/terms/> .
@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pav: <http://purl.org/pav/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix belv: <http://www.selventa.com/vocabulary/> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix chebi: <http://www.ebi.ac.uk/chebi/searchId.do?chebiId=> .
@prefix RNA: <http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_33697> .
@prefix rgd: <http://rgd.mcw.edu/rgdweb/report/gene/main.html?id=> .
@prefix geneProductOf: <http://purl.obolibrary.org/obo/RO_0002204> .
@prefix obo: <http://purl.obolibrary.org/obo/> .
@prefix occursIn: <http://purl.obolibrary.org/obo/BFO_0000066> .
@prefix species: <http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=> .
@prefix pubmed: <http://www.ncbi.nlm.nih.gov/pubmed/> .
@prefix orcid: <http://orcid.org/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubinfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:_1 geneProductOf: rgd:628703;
    a RNA: .
  
  sub:_2 occursIn: obo:CLO_0002140, obo:CL_0000127, species:10116;
    rdf:object sub:_1;
    rdf:predicate belv:increases;
    rdf:subject chebi:9252;
    a rdf:Statement .
  
  sub:assertion rdfs:label "a(CHEBI:staurosporine) -> r(RGD:Baalc)" .
}

sub:provenance {
  beldoc: dce:description "Approximately 61,000 statements.";
    dce:rights "Copyright (c) 2011-2012, Selventa. All rights reserved.";
    dce:title "BEL Framework Large Corpus Document";
    pav:authoredBy sub:_4;
    pav:version "20131211" .
  
  sub:_3 prov:value "The differential expression of the three genes was confirmed by RT-PCR using their respective gene-specific primers. cAMP-dependent phosphodiesterase shows about 4.5-fold increase in RNA from dbcAMP-treated astrocytes using a template amount of 10 ng, confirming the differential expression (Fig. 2). For BAALC, about 1.9-fold increase was seen in RNA from dbcAMP-treated astrocytes as compared to the control RNA (Fig. 3A) using a template amount of 20 ng. The expression of BAALC was also analyzed by RT-PCR in C6 glioma cells (Fig. 3B) treated with the differentiation inducing agents, viz. dbcAMP (1 mM), and staurosporine (1 nM). BAALC was found to be up regulated in dbc-treated C6 cells (9.4-fold) and to less extent in staurosporine-treated cells (1.7-fold). Up regulation of BAALC expression is thus observed both in normal and malignant astrocytes on differentiation. RT-PCR analysis demonstrated that RAMP3 expression is about 6.3-fold in control (DMSO) treated astrocytes as compared to those treated with staurosporine with a template amount of 10 ng (Fig. 4).";
    prov:wasQuotedFrom pubmed:16376586 .
  
  sub:_4 rdfs:label "Selventa" .
  
  sub:assertion prov:hadPrimarySource pubmed:16376586;
    prov:wasDerivedFrom beldoc:, sub:_3 .
}

sub:pubinfo {
  this: dct:created "2014-07-03T14:32:54.286+02:00"^^xsd:dateTime;
    pav:createdBy orcid:0000-0001-6818-334X, orcid:0000-0002-1267-0234 .
}