[ { "@graph" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdbdqqhmT70oxtPpUr-bbOzgcSxlgqcmluHHV1q1iHKs", "@type" : [ "http://www.nanopub.org/nschema#Nanopublication" ], "http://www.nanopub.org/nschema#hasAssertion" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdbdqqhmT70oxtPpUr-bbOzgcSxlgqcmluHHV1q1iHKs#assertion" } ], "http://www.nanopub.org/nschema#hasProvenance" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdbdqqhmT70oxtPpUr-bbOzgcSxlgqcmluHHV1q1iHKs#provenance" } ], "http://www.nanopub.org/nschema#hasPublicationInfo" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdbdqqhmT70oxtPpUr-bbOzgcSxlgqcmluHHV1q1iHKs#pubinfo" } ] } ], "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdbdqqhmT70oxtPpUr-bbOzgcSxlgqcmluHHV1q1iHKs#Head" }, { "@graph" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdbdqqhmT70oxtPpUr-bbOzgcSxlgqcmluHHV1q1iHKs#_1", "@type" : [ "http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_33697" ], "http://purl.obolibrary.org/obo/RO_0002204" : [ { "@id" : "http://rgd.mcw.edu/rgdweb/report/gene/main.html?id=628703" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdbdqqhmT70oxtPpUr-bbOzgcSxlgqcmluHHV1q1iHKs#_2", "@type" : [ "http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement" ], "http://purl.obolibrary.org/obo/BFO_0000066" : [ { "@id" : "http://purl.obolibrary.org/obo/CLO_0002140" }, { "@id" : "http://purl.obolibrary.org/obo/CL_0000127" }, { "@id" : "http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10116" } ], "http://www.w3.org/1999/02/22-rdf-syntax-ns#object" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdbdqqhmT70oxtPpUr-bbOzgcSxlgqcmluHHV1q1iHKs#_1" } ], "http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate" : [ { "@id" : "http://www.selventa.com/vocabulary/increases" } ], "http://www.w3.org/1999/02/22-rdf-syntax-ns#subject" : [ { "@id" : "http://www.ebi.ac.uk/chebi/searchId.do?chebiId=9252" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdbdqqhmT70oxtPpUr-bbOzgcSxlgqcmluHHV1q1iHKs#assertion", "http://www.w3.org/2000/01/rdf-schema#label" : [ { "@value" : "a(CHEBI:staurosporine) -> r(RGD:Baalc)" } ] } ], "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdbdqqhmT70oxtPpUr-bbOzgcSxlgqcmluHHV1q1iHKs#assertion" }, { "@graph" : [ { "@id" : "http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel", "http://purl.org/dc/elements/1.1/description" : [ { "@value" : "Approximately 61,000 statements." } ], "http://purl.org/dc/elements/1.1/rights" : [ { "@value" : "Copyright (c) 2011-2012, Selventa. All rights reserved." } ], "http://purl.org/dc/elements/1.1/title" : [ { "@value" : "BEL Framework Large Corpus Document" } ], "http://purl.org/pav/authoredBy" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdbdqqhmT70oxtPpUr-bbOzgcSxlgqcmluHHV1q1iHKs#_4" } ], "http://purl.org/pav/version" : [ { "@value" : "20131211" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdbdqqhmT70oxtPpUr-bbOzgcSxlgqcmluHHV1q1iHKs#_3", "http://www.w3.org/ns/prov#value" : [ { "@value" : "The differential expression of the three genes was confirmed by RT-PCR using their respective gene-specific primers. cAMP-dependent phosphodiesterase shows about 4.5-fold increase in RNA from dbcAMP-treated astrocytes using a template amount of 10 ng, confirming the differential expression (Fig. 2). For BAALC, about 1.9-fold increase was seen in RNA from dbcAMP-treated astrocytes as compared to the control RNA (Fig. 3A) using a template amount of 20 ng. The expression of BAALC was also analyzed by RT-PCR in C6 glioma cells (Fig. 3B) treated with the differentiation inducing agents, viz. dbcAMP (1 mM), and staurosporine (1 nM). BAALC was found to be up regulated in dbc-treated C6 cells (9.4-fold) and to less extent in staurosporine-treated cells (1.7-fold). Up regulation of BAALC expression is thus observed both in normal and malignant astrocytes on differentiation. RT-PCR analysis demonstrated that RAMP3 expression is about 6.3-fold in control (DMSO) treated astrocytes as compared to those treated with staurosporine with a template amount of 10 ng (Fig. 4)." } ], "http://www.w3.org/ns/prov#wasQuotedFrom" : [ { "@id" : "http://www.ncbi.nlm.nih.gov/pubmed/16376586" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdbdqqhmT70oxtPpUr-bbOzgcSxlgqcmluHHV1q1iHKs#_4", "http://www.w3.org/2000/01/rdf-schema#label" : [ { "@value" : "Selventa" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdbdqqhmT70oxtPpUr-bbOzgcSxlgqcmluHHV1q1iHKs#assertion", "http://www.w3.org/ns/prov#hadPrimarySource" : [ { "@id" : "http://www.ncbi.nlm.nih.gov/pubmed/16376586" } ], "http://www.w3.org/ns/prov#wasDerivedFrom" : [ { "@id" : "http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel" }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdbdqqhmT70oxtPpUr-bbOzgcSxlgqcmluHHV1q1iHKs#_3" } ] } ], "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdbdqqhmT70oxtPpUr-bbOzgcSxlgqcmluHHV1q1iHKs#provenance" }, { "@graph" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdbdqqhmT70oxtPpUr-bbOzgcSxlgqcmluHHV1q1iHKs", "http://purl.org/dc/terms/created" : [ { "@type" : "http://www.w3.org/2001/XMLSchema#dateTime", "@value" : "2014-07-03T14:32:54.286+02:00" } ], "http://purl.org/pav/createdBy" : [ { "@id" : "http://orcid.org/0000-0001-6818-334X" }, { "@id" : "http://orcid.org/0000-0002-1267-0234" } ] } ], "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdbdqqhmT70oxtPpUr-bbOzgcSxlgqcmluHHV1q1iHKs#pubinfo" } ]