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All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAdZJh74VVWXkXgo_DPnLgWskbAsNmCdo9Tt2O_X33BZg#_5 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RAdZJh74VVWXkXgo_DPnLgWskbAsNmCdo9Tt2O_X33BZg#_4 http://www.w3.org/ns/prov#value To confirm the in vivo interaction between the miR-15a/miR-16-1 cluster and Bcl-2 in NB4 cells, we transfected the precursor miR-16- 1 (pre-miR-16-1) oligonucleotide or scrambled oligonucleotides into NB4 cells by using nucleoporation and obtained total RNA at 24 h and proteins from whole cell lysates at 48 h. As shown in Figure 5c, there was a significant reduction of the Bcl-2 transcripts by qRT– PCR after transfection with pre-miR-16-1. Likewise, transfection of the pre-miR-16-1, but not the control, resulted in a strong Bcl-2 protein reduction by Western blots at 48 h (Figure 5e). http://www.tkuhn.ch/bel2nanopub/RAdZJh74VVWXkXgo_DPnLgWskbAsNmCdo9Tt2O_X33BZg#_4 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/17260024 http://www.tkuhn.ch/bel2nanopub/RAdZJh74VVWXkXgo_DPnLgWskbAsNmCdo9Tt2O_X33BZg#_5 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAdZJh74VVWXkXgo_DPnLgWskbAsNmCdo9Tt2O_X33BZg#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/17260024 http://www.tkuhn.ch/bel2nanopub/RAdZJh74VVWXkXgo_DPnLgWskbAsNmCdo9Tt2O_X33BZg#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAdZJh74VVWXkXgo_DPnLgWskbAsNmCdo9Tt2O_X33BZg#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAdZJh74VVWXkXgo_DPnLgWskbAsNmCdo9Tt2O_X33BZg#_4 http://www.tkuhn.ch/bel2nanopub/RAdZJh74VVWXkXgo_DPnLgWskbAsNmCdo9Tt2O_X33BZg#pubinfo http://www.tkuhn.ch/bel2nanopub/RAdZJh74VVWXkXgo_DPnLgWskbAsNmCdo9Tt2O_X33BZg http://purl.org/dc/terms/created 2014-07-03T14:33:08.298+02:00 http://www.tkuhn.ch/bel2nanopub/RAdZJh74VVWXkXgo_DPnLgWskbAsNmCdo9Tt2O_X33BZg http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAdZJh74VVWXkXgo_DPnLgWskbAsNmCdo9Tt2O_X33BZg http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234