sub:provenance {
  beldoc: dce:description "Approximately 61,000 statements." ;
    dce:rights "Copyright (c) 2011-2012, Selventa. All rights reserved." ;
    dce:title "BEL Framework Large Corpus Document" ;
    pav:authoredBy sub:_5 ;
    pav:version "20131211" .
  sub:_4 prov:value "To confirm the in vivo interaction between the miR-15a/miR-16-1 cluster and Bcl-2 in NB4 cells, we transfected the precursor miR-16- 1 (pre-miR-16-1) oligonucleotide or scrambled oligonucleotides into NB4 cells by using nucleoporation and obtained total RNA at 24 h and proteins from whole cell lysates at 48 h. As shown in Figure 5c, there was a significant reduction of the Bcl-2 transcripts by qRT– PCR after transfection with pre-miR-16-1. Likewise, transfection of the pre-miR-16-1, but not the control, resulted in a strong Bcl-2 protein reduction by Western blots at 48 h (Figure 5e)." ;
    prov:wasQuotedFrom pubmed:17260024 .
  sub:_5 rdfs:label "Selventa" .
  sub:assertion prov:hadPrimarySource pubmed:17260024 ;
    prov:wasDerivedFrom beldoc: , sub:_4 .
}