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[As sequencing technologies have become more sophisticated and have revealed an ever-increasing complexity of the genetic landscape of melanoma, it has become clear that sequencing methods applied to clinical specimens have to reliably capture not only recurrent 'hotspot' mutations like BRAFV600 and NRASQ61 or 'mini-hotspot' mutations like exon 11 and 13 c-KIT but also heterogeneous somatic mutations dispersed across multiple functionally conserved regions of genes or entire genes.]. Sentence from MEDLINE/PubMed, a database of the U.S. National Library of Medicine.
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