sub:provenance {
  beldoc: dce:description "Approximately 61,000 statements." ;
    dce:rights "Copyright (c) 2011-2012, Selventa. All rights reserved." ;
    dce:title "BEL Framework Large Corpus Document" ;
    pav:authoredBy sub:_6 ;
    pav:version "20131211" .
  sub:_5 prov:value "co-localization of hA1AT and active caspase-3 by immunofluorescence microscopy (Figure 3a) suggested an interaction between the hA1AT with the proapoptotic cysteine protease caspase-3 in mouse and rat microvascular pulmonary endothelial cells treated with staurosporine.....was further tested biochemically as mouse lung endothelial cells pretreated with hA1AT and exposed to staurosporine were evaluated by immunoprecipitation with active caspase-3 antibodies followed by immunoblotting with hA1AT-specific antibodies. This study confirmed an interaction between hA1AT and caspase-3 (Figure 3b) in lung primary endothelial cells....A complex containing hA1AT and caspase-3 was detected by incubating hA1AT with His-tagged caspase-3 for 1 hour, followed by electrophoresis and immunoblotting sequentially for A1AT and then His-tag (Figure 4a)." ;
    prov:wasQuotedFrom pubmed:17003475 .
  sub:_6 rdfs:label "Selventa" .
  sub:assertion prov:hadPrimarySource pubmed:17003475 ;
    prov:wasDerivedFrom beldoc: , sub:_5 .
}