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@prefix schema: <http://schema.org/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
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@prefix bibo: <http://purl.org/ontology/bibo/> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix foaf: <http://xmlns.com/foaf/0.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubinfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.org/coloc-seq/kinetics-model> schema:description "Mathematical model describing transcript protection dynamics with RNase gradient treatment"@en;
    schema:formula "f(A) = (1 - P0) * exp(-k'i * A) + P0"@en;
    schema:name "CoLoC-seq Kinetics Model"@en;
    sio:SIO_000211 <http://purl.org/coloc-seq/parameter-P0>, <http://purl.org/coloc-seq/parameter-k-prime>;
    a schema:Thing;
    rdfs:comment "Parameters P0 and k'i capture the proportion of protected RNA and digestion rate constant respectively"@en .
  
  <http://purl.org/coloc-seq/parameter-P0> schema:comment "P0=0: no protection (dissolved/disrupted membranes); P0=partial: partial protection (surface-attached); P0=1: full protection (genuine organellar localization)"@en;
    schema:description "Proportion of RNA protected from RNase degradation in the CoLoC-seq kinetics model"@en;
    schema:name "Protected pool parameter P0"@en;
    sio:SIO_000228 "0 to 1"@en;
    a schema:Thing .
  
  <http://purl.org/coloc-seq/parameter-k-prime> schema:comment "Fitted using Levenberg-Marquardt optimization; reflects differential susceptibility to RNase between transcript populations"@en;
    schema:description "Pseudo-first-order rate constant describing RNA degradation velocity"@en;
    schema:name "Digestion rate constant k'i"@en;
    a schema:Thing .
  
  this: schema:about <https://doi.org/10.1093/nar/gkac1205>;
    schema:text "The CoLoC-seq kinetics model uses the protected pool parameter P0 to distinguish genuinely localised organellar transcripts from surface-attached contaminants based on their degradation dynamics."@en;
    a <https://w3id.org/np/RA_C-L7pLBKSyHDhS2YIFmqfb_-BLGOldcUb8emWvRltA/o/ntemplate/AIDA-Sentence>;
    rdfs:seeAlso <http://purl.org/coloc-seq/method> .
}

sub:provenance {
  <https://doi.org/10.1093/nar/gkac1205> bibo:doi "10.1093/nar/gkac1205";
    schema:datePublished "2023"^^xsd:gYear;
    schema:isPartOf sub:__1;
    schema:name "CoLoC-seq probes the global topology of organelle transcriptomes"@en;
    a bibo:AcademicArticle, schema:ScholarlyArticle .
  
  sub:__1 schema:issn "0305-1048";
    schema:name "Nucleic Acids Research"@en;
    a schema:Periodical .
  
  sub:assertion prov:wasAttributedTo orcid:0000-0001-8763-6703, orcid:0000-0002-1481-2996,
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    prov:wasDerivedFrom <https://doi.org/10.1093/nar/gkac1205> .
}

sub:pubinfo {
  orcid:0009-0005-7752-8203 foaf:name "Tiado Kollender" .
  
  this: dct:created "2026-01-11T00:00:00Z"^^xsd:dateTime;
    dct:license <https://creativecommons.org/publicdomain/zero/1.0/>;
    npx:signedBy orcid:0009-0005-7752-8203;
    npx:wasCreatedFromPubinfoTemplate <https://w3id.org/np/RA0J4vUn_dekg-U1kK3AOEt02p9mT2WO03uGxLDec1jLw>,
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    pav:createdBy orcid:0009-0005-7752-8203;
    a npx:ExampleNanopub;
    rdfs:comment "AIDA-based assertion explaining the mathematical model and key parameters of CoLoC-seq"@en;
    rdfs:label "Nanopublication: CoLoC-seq kinetics model and protected pool parameter"@en;
    prov:wasAttributedTo orcid:0009-0005-7752-8203 .
  
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