. . . . "Mathematical model describing transcript protection dynamics with RNase gradient treatment"@en . "f(A) = (1 - P0) * exp(-k'i * A) + P0"@en . "CoLoC-seq Kinetics Model"@en . . . . "Parameters P0 and k'i capture the proportion of protected RNA and digestion rate constant respectively"@en . "P0=0: no protection (dissolved/disrupted membranes); P0=partial: partial protection (surface-attached); P0=1: full protection (genuine organellar localization)"@en . "Proportion of RNA protected from RNase degradation in the CoLoC-seq kinetics model"@en . "Protected pool parameter P0"@en . "0 to 1"@en . . "Fitted using Levenberg-Marquardt optimization; reflects differential susceptibility to RNase between transcript populations"@en . "Pseudo-first-order rate constant describing RNA degradation velocity"@en . "Digestion rate constant k'i"@en . . . "The CoLoC-seq kinetics model uses the protected pool parameter P0 to distinguish genuinely localised organellar transcripts from surface-attached contaminants based on their degradation dynamics."@en . . . "10.1093/nar/gkac1205" . "2023"^^ . . "CoLoC-seq probes the global topology of organelle transcriptomes"@en . . . "0305-1048" . "Nucleic Acids Research"@en . . . . . . "Tiado Kollender" . "2026-01-11T00:00:00Z"^^ . . . . . . . "AIDA-based assertion explaining the mathematical model and key parameters of CoLoC-seq"@en . "Nanopublication: CoLoC-seq kinetics model and protected pool parameter"@en . . "RSA" . "MIIBIjANBgkqhkiG9w0BAQEFAAOCAQ8AMIIBCgKCAQEAoSR/rqhnkTPQO8u4l2JQAemJo5cN83rT54nA5NXHUqaeu26bEjT/rsX1PCVBhhJ3cV2uu4wjI0NfcVaKPKmODSf6p579tdOykOnjbWLbxxPMe5DC5eV1ThMHsFsNU/6vCYIfS00E7wre7fnFYz7FAG7v1MZEyWeSXsHgk9jHoqQQDPqup5PHReKp1dd6J0Nht/OfSYnUancKAgIFyPhkTlThsLK5xSNyNEgiTR7pwkP5pGq2AnD6bPw4G3kiPnIpIGZNsaeIOvhn64gwbrtoJVdoun/namw6FQ7StEC5km/0evaBZc4qIextwO/s32Wru77ukblJ0Xi/YzbC0iLuIQIDAQAB" . "dPCr0Fzp6tHRo4Iqfso3+lxfNvDyLzmTuUlB3gGFm1eVro8E72BACvNYGh+5IKB26K2lwfgzNs8IQzzFvJLuEck+tYVFkxlo5r6NSk3TJn9OevVCreeHwy0ldOm9aNMDz/XIClGzN1jLbczXbgVA4rKdJh1DNaPdMw8svoQKsJqYzEAjR1PEOHpdnM9ON9XnVGlosEZEAePG/UG2LnLHrq6Ff/l7e825rbAnZ/O4ALc3NWzOgMOzbNjVnBB/PETm3TpgmCR2Q9Dqni3wG3ewFjRVyA336eBEN+0+BOVOwBx0Td+Jh+AUlLlF9q+yel/3pUBvkzcfCNKkmGuXg+8GgQ==" . .