sub:assertion {
  <http://purl.org/coloc-seq/kinetics-model> schema:description "Mathematical model describing transcript protection dynamics with RNase gradient treatment"@en ;
    schema:formula "f(A) = (1 - P0) * exp(-k'i * A) + P0"@en ;
    schema:name "CoLoC-seq Kinetics Model"@en ;
    sio:SIO_000211 <http://purl.org/coloc-seq/parameter-P0> , <http://purl.org/coloc-seq/parameter-k-prime> ;
    a schema:Thing ;
    rdfs:comment "Parameters P0 and k'i capture the proportion of protected RNA and digestion rate constant respectively"@en .
  <http://purl.org/coloc-seq/parameter-P0> schema:comment "P0=0: no protection (dissolved/disrupted membranes); P0=partial: partial protection (surface-attached); P0=1: full protection (genuine organellar localization)"@en ;
    schema:description "Proportion of RNA protected from RNase degradation in the CoLoC-seq kinetics model"@en ;
    schema:name "Protected pool parameter P0"@en ;
    sio:SIO_000228 "0 to 1"@en ;
    a schema:Thing .
  <http://purl.org/coloc-seq/parameter-k-prime> schema:comment "Fitted using Levenberg-Marquardt optimization; reflects differential susceptibility to RNase between transcript populations"@en ;
    schema:description "Pseudo-first-order rate constant describing RNA degradation velocity"@en ;
    schema:name "Digestion rate constant k'i"@en ;
    a schema:Thing .
  this: schema:about <https://doi.org/10.1093/nar/gkac1205> ;
    schema:text "The CoLoC-seq kinetics model uses the protected pool parameter P0 to distinguish genuinely localised organellar transcripts from surface-attached contaminants based on their degradation dynamics."@en ;
    a <https://w3id.org/np/RA_C-L7pLBKSyHDhS2YIFmqfb_-BLGOldcUb8emWvRltA/o/ntemplate/AIDA-Sentence> ;
    rdfs:seeAlso <http://purl.org/coloc-seq/method> .
}