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All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAZsMwNdPJJA81Jo9FFrtlzJzDSnvsf49of7PoylK2GlU#_3 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RAZsMwNdPJJA81Jo9FFrtlzJzDSnvsf49of7PoylK2GlU#_2 http://www.w3.org/ns/prov#value mitochondria-enriched fractions prepared from these cells were lysed and subjected to SDS-PAGE. Even if mtNOS is constitutively expressed, the levels seem to be low, since no iNOS immunoreactivity was detected in the absence of LPS and under experimental conditions used (Figure ?(Figure6C).6C). However, in the mitochondrial fraction prepared from LPS-exposed ECs, mtNOS protein expression is easily detected.....mtNOS activity was assessed by exposing freshly prepared, unlysed mitochondrial fractions from ECs to oxyHb, as described under Methods. The rate of NO production was measured by the change in optical density of the reaction mixture as generated NO quickly oxidized oxyHb to metHb. 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