. . . . "Distinguishes genuinely resident transcripts from slowly degradable contaminants"@en . "Tracks depletion dynamics across gradient instead of single RNase treatment"@en . "Enzymatic Kinetics-Based Approach"@en . . "Overcomes major limitation of standard fractionation methods"@en . "P0 = 0 for all RNAs when membranes are dissolved"@en . "Mock CoLoC-seq Control Experiment"@en . "Control for false-positive identifications"@en . "Distinguish organellar protection from intrinsic RNase resistance"@en . . "Essential quality control for validating resident RNA identification"@en . "Applicable to any cell type or tissue"@en . "Broader applicability than proximity labeling methods"@en . "Can be implemented in non-model species"@en . "No Genetic Modification Required"@en . . "Key practical advantage for diverse research applications"@en . "Compatible with non-model organisms"@en . "No genetic modification required"@en . "Extracellular vesicles"@en . "Mitochondria"@en . "Plastids"@en . "Vacuolar compartments"@en . "Viral particles"@en . "Universal Applicability"@en . . "Major advantage over proximity labeling techniques"@en . . . . . "CoLoC-seq advances subcellular transcriptomics by combining enzymatic kinetics with Mock control experiments to overcome false-positive limitations of conventional fractionation methods and eliminating the requirement for genetic modification."@en . . . "10.1093/nar/gkac1205" . "2023"^^ . . "CoLoC-seq probes the global topology of organelle transcriptomes"@en . . "Nucleic Acids Research"@en . . . . . . . . . "Tiado Kollender" . "2026-01-11T00:00:00Z"^^ . . . . . . "AIDA-based assertion about CoLoC-seq innovations compared to existing subcellular transcriptomics methods"@en . "Nanopublication: CoLoC-seq design innovations and advantages"@en . . "RSA" . "MIIBIjANBgkqhkiG9w0BAQEFAAOCAQ8AMIIBCgKCAQEAoSR/rqhnkTPQO8u4l2JQAemJo5cN83rT54nA5NXHUqaeu26bEjT/rsX1PCVBhhJ3cV2uu4wjI0NfcVaKPKmODSf6p579tdOykOnjbWLbxxPMe5DC5eV1ThMHsFsNU/6vCYIfS00E7wre7fnFYz7FAG7v1MZEyWeSXsHgk9jHoqQQDPqup5PHReKp1dd6J0Nht/OfSYnUancKAgIFyPhkTlThsLK5xSNyNEgiTR7pwkP5pGq2AnD6bPw4G3kiPnIpIGZNsaeIOvhn64gwbrtoJVdoun/namw6FQ7StEC5km/0evaBZc4qIextwO/s32Wru77ukblJ0Xi/YzbC0iLuIQIDAQAB" . "AEcAJ6oJVhJfoC+rwTDoB14K+4H9SwrTO4QiGZvk7hmxIEA6aJ+MRZ2x6vfHAjZJj0fwhwgWRxWiVkTpZ9/IdnbTxdY7DvMGrVUBZVHwOMeYTPt+hopVGUA0mzrnXnjYqswfOWTDpLV4JQ1GzlJSj1bGUhmf64yyckFNJvfayyT679qCHYxhcyIuMbSZgovu5mdowThvWf0uP7JjDVhlAdatlx0wtwDPOisrFqqSJQUYee8zObb8+WyLXIjwofxqyFf7owtpjavzBIpEUdHOY/Kstnfrw/rd1+xPFgqtbc1EKk6oDciFi/fV49s+8ZgAGbBqAvd+wi/LkmMQ5bUIlQ==" . .