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@prefix edam: <http://edamontology.org/> .
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@prefix bibo: <http://purl.org/ontology/bibo/> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix foaf: <http://xmlns.com/foaf/0.1/> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubinfo ;
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}
sub:assertion {
   <http://purl.org/coloc-seq/advantage-kinetics-approach> schema:advantage "Distinguishes genuinely resident transcripts from slowly degradable contaminants"@en ;
    schema:description "Tracks depletion dynamics across gradient instead of single RNase treatment"@en ;
    schema:name "Enzymatic Kinetics-Based Approach"@en ;
    a schema:Thing ;
    rdfs:comment "Overcomes major limitation of standard fractionation methods"@en .
  <http://purl.org/coloc-seq/advantage-mock-control> schema:expectedResult "P0 = 0 for all RNAs when membranes are dissolved"@en ;
    schema:name "Mock CoLoC-seq Control Experiment"@en ;
    schema:purpose "Control for false-positive identifications"@en , "Distinguish organellar protection from intrinsic RNase resistance"@en ;
    a schema:Thing ;
    rdfs:comment "Essential quality control for validating resident RNA identification"@en .
  <http://purl.org/coloc-seq/advantage-no-genetic-modification> schema:benefit "Applicable to any cell type or tissue"@en , "Broader applicability than proximity labeling methods"@en , "Can be implemented in non-model species"@en ;
    schema:name "No Genetic Modification Required"@en ;
    a schema:Thing ;
    rdfs:comment "Key practical advantage for diverse research applications"@en .
  <http://purl.org/coloc-seq/advantage-universal-applicability> schema:advantage "Compatible with non-model organisms"@en , "No genetic modification required"@en ;
    schema:applicableTo "Extracellular vesicles"@en , "Mitochondria"@en , "Plastids"@en , "Vacuolar compartments"@en , "Viral particles"@en ;
    schema:name "Universal Applicability"@en ;
    a schema:Thing ;
    rdfs:comment "Major advantage over proximity labeling techniques"@en .
  this: npx:relatesTo <https://w3id.org/np/RAMVUimnATifJYJw0VP2zAesw4OK_Gw30p9NgcEU4oVW8> , <https://w3id.org/np/RA_C-L7pLBKSyHDhS2YIFmqfb_-BLGOldcUb8emWvRltA> , <https://w3id.org/np/RAqWgWTy_lLfUrvdgqP0lggMHH_Of_5TgmpDWkpxe16gg> ;
    schema:about <https://doi.org/10.1093/nar/gkac1205> ;
    schema:text "CoLoC-seq advances subcellular transcriptomics by combining enzymatic kinetics with Mock control experiments to overcome false-positive limitations of conventional fractionation methods and eliminating the requirement for genetic modification."@en ;
    a <https://w3id.org/np/RAZTGowlvcV7tu_Gi1DpIhq3KNCra6KQBgpAdqx7gSMrE/o/ntemplate/AIDA-Sentence> ;
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sub:provenance {
   <https://doi.org/10.1093/nar/gkac1205> bibo:doi "10.1093/nar/gkac1205" ;
    schema:datePublished "2023"^^xsd:gYear ;
    schema:isPartOf sub:__1 ;
    schema:name "CoLoC-seq probes the global topology of organelle transcriptomes"@en ;
    a schema:ScholarlyArticle .
  sub:__1 schema:name "Nucleic Acids Research"@en ;
    a schema:Periodical .
  sub:assertion prov:wasAttributedTo orcid:0000-0001-8763-6703 , orcid:0000-0002-1481-2996 , orcid:0000-0002-9142-7979 ;
    prov:wasDerivedFrom <https://doi.org/10.1093/nar/gkac1205> , <https://w3id.org/np/RAMVUimnATifJYJw0VP2zAesw4OK_Gw30p9NgcEU4oVW8> , <https://w3id.org/np/RA_C-L7pLBKSyHDhS2YIFmqfb_-BLGOldcUb8emWvRltA> , <https://w3id.org/np/RAqWgWTy_lLfUrvdgqP0lggMHH_Of_5TgmpDWkpxe16gg> .
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sub:pubinfo {
   orcid:0009-0005-7752-8203 foaf:name "Tiado Kollender" .
  this: dct:created "2026-01-11T00:00:00Z"^^xsd:dateTime ;
    dct:license <https://creativecommons.org/publicdomain/zero/1.0/> ;
    npx:wasCreatedFromPubinfoTemplate <https://w3id.org/np/RA0J4vUn_dekg-U1kK3AOEt02p9mT2WO03uGxLDec1jLw> , <https://w3id.org/np/RAukAcWHRDlkqxk7H2XNSegc1WnHI569INvNr-xdptDGI> ;
    pav:createdBy orcid:0009-0005-7752-8203 ;
    a npx:ExampleNanopub ;
    rdfs:comment "AIDA-based assertion about CoLoC-seq innovations compared to existing subcellular transcriptomics methods"@en ;
    rdfs:label "Nanopublication: CoLoC-seq design innovations and advantages"@en ;
    prov:wasAttributedTo orcid:0009-0005-7752-8203 .
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