http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#Head http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4 http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#assertion http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4 http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#provenance http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4 http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#pubinfo http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#assertion http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#_1 http://semanticscience.org/resource/SIO_000139 http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#_2 http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://amigo.geneontology.org/amigo/term/GO:0042789 http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#_2 http://purl.obolibrary.org/obo/RO_0002204 http://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=9984 http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_36080 http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#_3 http://purl.obolibrary.org/obo/RO_0002204 http://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=6835 http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#_3 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_33697 http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#_4 http://purl.obolibrary.org/obo/BFO_0000066 http://purl.obolibrary.org/obo/CLO_0004283 http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#_4 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606 http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#_4 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#_3 http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#_4 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/decreases http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#_4 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#_1 http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#_4 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#assertion http://www.w3.org/2000/01/rdf-schema#label tscript(p(HGNC:RFX3)) -| r(HGNC:MAP1A) http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#provenance http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#_6 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#_5 http://www.w3.org/ns/prov#value Both RFX1 and RFX3 Down-regulate the MAP1A Promoter in Non-neuronal Cells-- Because EMSA experiments suggested that RFX1 and 3 are the major proteins binding to the RFX sites in the MAP1A gene, we examined their functional effects on the promoter activity in reporter assay. We made RFX1 and RFX3 expression constructs driven by the CMV promoter. The expression of proteins in expected sizes and appropriate nuclear localizations of EGFP-tagged proteins were confirmed by Western blotting and confocal microscopy, respectively (data not shown). We then examined their effects on the hMP1-8 reporter activity in non-neuronal and neuronal cells (Fig. 6A). Overexpression of RFX1 resulted in down-regulation of the reporter compared with control in all non-neuronal cells examined (Fig. 6B, pFC R1). Reporter activity was also down-regulated when RFX 3 was overexpressed in HT29 and HEK293 cells (Fig. 6B, pFC R3). However, overexpression of RFX3 did not result in down-regulation, but slight up-regulation of the reporter in HeLa cells. http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#_5 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/12411430 http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#_6 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/12411430 http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#_5 http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4#pubinfo http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4 http://purl.org/dc/terms/created 2014-07-03T14:31:56.102+02:00 http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4 http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAZPphSkhO_fsi3jUFf--08ahtDNIVUylM0jYCigr4SD4 http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234