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All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAZOLGOMm6CJ1mAn_vjzSCRlAMQ_-mAGrz4nMjxFwrrPA#_4 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RAZOLGOMm6CJ1mAn_vjzSCRlAMQ_-mAGrz4nMjxFwrrPA#_3 http://www.w3.org/ns/prov#value FKLF-2 also activated all the other erythroid specific promoters we tested (GATA-1, glycophorin B, ferrochelatase, porphobilinogen deaminase, and 5-aminolevulinate synthase). To test whether FKLF-2 expression changes on induction of erythroid differentiation, K562, HEL, and MEL cells were induced by hemin, delta -ALA, or hemin + HMBA,26,27 and FKLF-2 mRNA was assessed with Northern blotting. As shown in Figure 2C and 2D, induction of erythroid differentiation up-regulated FKLF-2 expression in all 3 cell lines. 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