. . . . . . . . . . . . . . . "a(SCHEM:Sorbitol) -> kin(p(HGNC:MAPK8))" . "Approximately 61,000 statements." . "Copyright (c) 2011-2012, Selventa. All rights reserved." . "BEL Framework Large Corpus Document" . . "20131211" . "Hyperosmotic stress (0±5 M sorbitol) in combination with protein Ser}Thr phosphatase inhibition (0±2 lM okadaic acid) induced a prolonged activation of 46 and 54 kDa SAPKs in cytosolic extracts of ventricular myocytes (Figure 1A). The order of potency of SAPK activation was studied by the in-gel kinase assay. Cells were exposed to 0±5 M sorbitol (S), 0±5 M sorbitol­0±2 lM okadaic acid (SOA), 0±2 lM okadaic acid (OA) or 50 ng/ml anisomycin (A) for 30 min at 37 °C. Okadaic acid (0±2 lM) alone caused only a marginal stimulation but enhanced the response to sorbitol.SAPK activity was immunoprecipitated from nuclear extracts of cells exposed to 0±5 M sorbitol­0±2 lM okadaic acid using a JNK1 antibody (Figure 1C)." . . "Selventa" . . . . "2014-07-03T14:34:00.696+02:00"^^ . . .