sub:provenance {
  beldoc: dce:description "Approximately 61,000 statements." ;
    dce:rights "Copyright (c) 2011-2012, Selventa. All rights reserved." ;
    dce:title "BEL Framework Large Corpus Document" ;
    pav:authoredBy sub:_4 ;
    pav:version "20131211" .
  sub:_3 prov:value "Culturing the cells in lipoprotein-deficient serum for 2 days decreased their total cholesterol concentration by 25% (from 68 to 51ug cholesterol/mg protein) but did not significantly alter the ORP-1 mRNA levels. However, when the partially delipidated cells were loaded with LDL for 24 h, the ORP-1 transcript was up-regulated by 2-fold (Fig. 5). Simultaneously, the cellular cholesterol level returned back to normal (not shown). Short-term treatment of the sterol-depleted cells with 25-hydroxycholesterol (5 ug/ml of 25-hydroxycholesterol for 4 h), a condition known to stimulate the translocation of OSBP to the Golgi apparatus (10, 13), had a minor (1.5-fold) stimulatory effect on the expression of the ORP-1 mRNA (Fig. 5)." ;
    prov:wasQuotedFrom pubmed:10588946 .
  sub:_4 rdfs:label "Selventa" .
  sub:assertion prov:hadPrimarySource pubmed:10588946 ;
    prov:wasDerivedFrom beldoc: , sub:_3 .
}