http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#Head
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0
http://www.nanopub.org/nschema#hasAssertion
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#assertion
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0
http://www.nanopub.org/nschema#hasProvenance
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#provenance
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0
http://www.nanopub.org/nschema#hasPublicationInfo
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#pubInfo
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0
http://www.w3.org/1999/02/22-rdf-syntax-ns#type
http://www.nanopub.org/nschema#Nanopublication
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#assertion
http://purl.obolibrary.org/obo/OBI_0000136
http://www.w3.org/2000/01/rdf-schema#label
incubator
http://purl.obolibrary.org/obo/OBI_0400155
http://www.w3.org/2000/01/rdf-schema#label
water bath
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#step
http://purl.org/dc/elements/1.1/description
The falcon tube was kept into the 65°C incubator or water bath and mix gently by inversion after every 10 min till 45 min. After incubation, place the tube at room temperature for five min to reach to room temperature environment. Centrifuge the 50ml falcon tube for 5 min at 3000×g on room temperature. For next generation sequencing, the greater the genome size, lower is speed of initial centrifugation.
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#step
http://www.w3.org/1999/02/22-rdf-syntax-ns#type
http://purl.org/net/p-plan#Step
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed
http://purl.obolibrary.org/obo/OBI_0000136
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed
http://purl.obolibrary.org/obo/OBI_0400155
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed
http://www.bioassayontology.org/bao#BAO_0010014
http://www.bioassayontology.org/bao#BAO_0010014
http://www.w3.org/2000/01/rdf-schema#label
falcon tube
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#provenance
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#assertion
http://purl.org/pav/createdWith
https://nanotate.bitsfetch.com
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#assertion
http://www.w3.org/ns/prov#generatedAtTime
2021-02-25T16:27:40.948783
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#assertion
http://www.w3.org/ns/prov#wasAttributedTo
https://hypothes.is/users/zuluaga.lizzz
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#pubInfo
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/GopppHesEeujyW9es8rNpQ
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/MhH_dHesEeus53fiN4BdUw
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/U9JVgnesEeu3F8u9cIF0ow
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/a8EWxHesEeuIMa-qEOPYvw
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#sig
http://purl.org/nanopub/x/hasAlgorithm
RSA
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#sig
http://purl.org/nanopub/x/hasPublicKey
MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#sig
http://purl.org/nanopub/x/hasSignature
gKOlM9RQW/qsPKDjuVN+OHFnw7pero8K2dJcwpwEil0G8S0oEHO2AXBcCjgEkn+zQbtr0Dfz9hnhKm3WcstUpFE31fi7sgs4RC6MSUS7nv7y9TrDFPth+zst7gN0VAy9w9IJaXlhIJKFUMD3cIC/OfUgqdis58L242vP3Uippis=
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#sig
http://purl.org/nanopub/x/hasSignatureTarget
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0
http://purl.org/nanopub/x/introduces
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#step
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0
http://www.w3.org/ns/prov#generatedAtTime
2021-02-25T16:27:40.948783
http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0
http://www.w3.org/ns/prov#wasAttributedTo
https://hypothes.is/users/zuluaga.lizzz