http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#Head http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0 http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#assertion http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0 http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#provenance http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0 http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#pubinfo http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#assertion http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#_1 http://semanticscience.org/resource/SIO_000139 http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#_2 http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://amigo.geneontology.org/amigo/term/GO:0003824 http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#_2 http://purl.obolibrary.org/obo/RO_0002204 http://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=6677 http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_36080 http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#_3 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606 http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#_3 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://purl.bioontology.org/ontology/MSH/D050155 http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#_3 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/increases http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#_3 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#_1 http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#_3 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#assertion http://www.w3.org/2000/01/rdf-schema#label cat(p(HGNC:LPL)) -> bp(MESHPP:Lipogenesis) http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#provenance http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#_5 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#_4 http://www.w3.org/ns/prov#value The balance of lipid flux in adipocytes is controlled by the opposing actions of lipolysis and lipogenesis, which are controlled primarily by hormone-sensitive lipase and lipoprotein lipase (LPL), respectively. Catecholamines stimulate adipocyte lipolysis through reversible phosphorylation of hormone-sensitive lipase, and simultaneously inhibit LPL activity. However, LPL regulation is complex and previous studies have described translational regulation of LPL in response to catecholamines because of an RNA-binding protein that interacts with the 3'-untranslated region of LPL mRNA. In this study, we identified several protein components of an LPL RNA binding complex. Using an LPL RNA affinity column, we identified two of the RNA-binding proteins as the catalytic (C) subunit of cAMP-dependent protein kinase (PKA), and A kinase anchoring protein (AKAP) 121/149, one of the PKA anchoring proteins, which has known RNA binding activity. To determine whether the C subunit was involved in LPL translation inhibition, the C subunit was depleted from the cytoplasmic extract of epinephrine-stimulated adipocytes by immunoprecipitation. This resulted in the loss of LPL translation inhibition activity of the extract, along with decreased RNA binding activity in a gel shift assay. To demonstrate the importance of the AKAPs, inhibition of PKA-AKAP binding with a peptide competitor (HT31) prevented epinephrine-mediated inhibition of LPL translation. C subunit kinase activity was necessary for LPL RNA binding and translation inhibition, suggesting that the phosphorylation of AKAP121/149 or other proteins was an important part of RNA binding complex formation. The hormonal activation of PKA results in the reversible phosphorylation of hormone-sensitive lipase, which is the primary mediator of adipocyte lipolysis. These studies demonstrate a dual role for PKA to simultaneously inhibit LPL-mediated lipogenesis through inhibition of LPL translation. http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#_4 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/12218046 http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#_5 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/12218046 http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#_4 http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0#pubinfo http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0 http://purl.org/dc/terms/created 2014-07-03T14:31:53.827+02:00 http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0 http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RATAzuffXXrXFSmQcCUCt7OzhSQQ5KJ1ewO0sMtg2-kG0 http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234