http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#Head http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#assertion http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#provenance http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#pubinfo http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#assertion http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#_1 http://semanticscience.org/resource/SIO_000139 http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#_2 http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://amigo.geneontology.org/amigo/term/GO:0042789 http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#_2 http://purl.obolibrary.org/obo/RO_0002204 http://www.informatics.jax.org/marker/MGI:109340 http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_36080 http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#_3 http://purl.obolibrary.org/obo/RO_0002204 http://www.informatics.jax.org/marker/MGI:95582 http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#_3 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_33697 http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#_4 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10090 http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#_4 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#_3 http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#_4 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/directlyIncreases http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#_4 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#_1 http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#_4 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#assertion http://www.w3.org/2000/01/rdf-schema#label tscript(p(MGI:Pitx2)) => r(MGI:Fshb) http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#provenance http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#_6 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#_5 http://www.w3.org/ns/prov#value Here, we examined mechanisms through which the family of PITX proteins control murine FSH beta-subunit (Fshb) transcription. We observed that endogenous PITX1 and PITX2 isoforms from murine LbetaT2 gonadotrope cells could bind a highly conserved proximal cis-element. Transfection of PITX1 or PITX2C in heterologous cells stimulated both murine and human Fshb/FSHB promoter-reporter activities, and in both cases, mutation of the critical cis-element abrogated these effects.In homologous LbetaT2 cells, the same mutation decreased basal reporter activity and greatly reduced activin A-stimulated transcription from murine and human promoter-reporters. Transfecting dominant-negative forms of PITX1 or PITX2C or knocking down PITX1 or -2 expression by RNA interference in LbetaT2 cells inhibited murine Fshb transcription, confirming roles for endogenous PITX proteins. http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#_5 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/18339718 http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#_6 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/18339718 http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#_5 http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ#pubinfo http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ http://purl.org/dc/terms/created 2014-07-03T14:33:18.577+02:00 http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAS_G5AaHSyzcLSZ29JQcm-hREO8jNwNCXvm9oPIxCYkQ http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234