http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#Head http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#assertion http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#provenance http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#pubinfo http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#assertion http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#_1 http://purl.obolibrary.org/obo/RO_0002204 http://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=13253 http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_33697 http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://purl.obolibrary.org/obo/UBERON_0004237 http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606 http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#_1 http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/increases http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://www.ebi.ac.uk/chebi/searchId.do?chebiId=23965 http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#assertion http://www.w3.org/2000/01/rdf-schema#label a(CHEBI:estradiol) -> r(HGNC:CKLF) http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#provenance http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#_4 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#_3 http://www.w3.org/ns/prov#value Estrogen has been postulated to be involved in inhibition of vascular smooth muscle cell (VSMC) proliferation mainly via estrogen receptor (ER), but the detailed mechanism has remained primarily unknown. Therefore, in this study, microarray analysis was used in two types of cultured human VSMCs: one positive for ER alpha, and the other for ER beta, which were treated by estrogens to detect the estrogen-responsive genes. We also used quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) to evaluate mRNA levels of selective target gene (TG) in these cells. We further studied whether the TG product was involved in inhibition of proliferation using small interfering RNA (siRNA) of the TG transfection. We subsequently used quantitative RT-PCR and in situ hybridization analysis to evaluate the expression of these gene products in human aorta. E4F1, a possible inducer of cell growth arrest, was markedly increased only in ER alpha-positive VSMCs by estrogens in both microarray and RT-PCR analyses. Blocking of E4F1 using siRNA suppressed estrogenic inhibition of ER alpha-positive VSMC proliferation. E4F1 mRNA was abundant in premenopausal female aorta with mild atherosclerotic changes. E4F1 is therefore considered one of the estrogen-responsive genes involving ER alpha-mediated inhibition of VSMC proliferation and may play an important role in estrogen-related atheroprotection of human aorta. http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/15579445 http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/15579445 http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#_3 http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo#pubinfo http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo http://purl.org/dc/terms/created 2014-07-03T14:32:35.679+02:00 http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RARPGC7SmRn370Apt-tZO8faawjfeOKBlfV38Jvh2tUbo http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234