http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#Head http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#assertion http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#provenance http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#pubinfo http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#assertion http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#_1 http://purl.obolibrary.org/obo/RO_0002204 http://www.ncbi.nlm.nih.gov/gene/492938 http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_36080 http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://purl.bioontology.org/ontology/MSH/D006973 http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10090 http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#_1 http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/increases http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://resource.belframework.org/belframework/1.0/namespace/selventa-legacy-chemical-names/Sodium http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#assertion http://www.w3.org/2000/01/rdf-schema#label a(SCHEM:Sodium) -> p(EGID:492938) http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#provenance http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#_4 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#_3 http://www.w3.org/ns/prov#value BACKGROUND: Previous studies have indicated that catechol-O-methyltransferase (COMT) can modulate renal dopaminergic tone. OBJECTIVE: To test the hypothesis that COMT blockade protects from salt-induced hypertension. METHODS: COMT gene-disrupted (-/-) mice and wild-type controls received a high-sodium diet (NaCl 6%) for 3 weeks. Blood pressure and heart rate were recorded by radiotelemetry. Tissue and urine samples were assessed by light microscopy and high-performance liquid chromatography. The effects of nitecapone treatment were also examined. Systolic blood pressure and heart rate during normal sodium diet were similar in COMT (-/-) and wild-type mice. The high-sodium diet increased night-time systolic and diastolic blood pressures in wild-type mice, whereas blood pressure in COMT (-/-) mice remained unaltered. In wild-type mice, the sodium-induced increase in blood pressure was completely normalized by treatment with the COMT inhibitor, nitecapone. At baseline, 24-h urinary excretion of levodopa (L-DOPA), dopamine and noradrenaline was increased by 145, 85 and 74%, respectively, in COMT (-/-) mice compared with wild-type controls. In COMT (-/-) and wild-type mice, a high-sodium diet increased urinary L-DOPA excretion by 405 and 660% (reflected as 102 and 212% increases in dopamine excretion), respectively. The absolute amounts of urinary L-DOPA and dopamine remained 60 and 20% greater in COMT (-/-) mice. The high-sodium diet did not influence renal cortical COMT activity. CONCLUSION: Our findings suggest that COMT deficiency in mice increases the availability of L-DOPA, leading to enhanced dopaminergic tone, which may be associated with resistance to salt-induced hypertension. The findings of the present study also underline the importance of COMT in the regulation of blood pressure, sodium excretion and renal dopaminergic tone. http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/14654758 http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/14654758 http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#_3 http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw#pubinfo http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw http://purl.org/dc/terms/created 2014-07-03T14:30:18.818+02:00 http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAOTR4TYqlNIAPF5-ukIDJEob-nyemwIAtjG6dCZk96rw http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234