@prefix dcterms: . @prefix this: . @prefix sub: . @prefix beldoc: . @prefix rdfs: . @prefix rdf: . @prefix xsd: . @prefix dce: . @prefix pav: . @prefix np: . @prefix belv: . @prefix prov: . @prefix go: . @prefix Protein: . @prefix hgnc: . @prefix geneProductOf: . @prefix species: . @prefix occursIn: . @prefix obo: . @prefix pubmed: . @prefix orcid: . sub:Head { this: np:hasAssertion sub:assertion; np:hasProvenance sub:provenance; np:hasPublicationInfo sub:pubinfo; a np:Nanopublication . } sub:assertion { sub:_1 belv:translocationFrom go:0005737; belv:translocationOf sub:_2; belv:translocationTo go:0005730 . sub:_2 geneProductOf: hgnc:6968; a Protein: . sub:_3 occursIn: obo:UBERON_0004852, species:9606; rdf:object sub:_1; rdf:predicate belv:increases; rdf:subject go:0009408; a rdf:Statement . sub:assertion rdfs:label "bp(GOBP:\"response to heat\") -> tloc(p(HGNC:DNAJB9),GOCCID:0005737,GOCCID:0005730)" . } sub:provenance { beldoc: dce:description "Approximately 61,000 statements."; dce:rights "Copyright (c) 2011-2012, Selventa. All rights reserved."; dce:title "BEL Framework Large Corpus Document"; pav:authoredBy sub:_5; pav:version "20131211" . sub:_4 prov:value "To evaluate whether Mdg1 actually accumulated within the nucleoli, HeLa cells were transfected with pEGFP-Mdg1, heat shocked, and immunostained with a nucleolus-specific antibody. Red-fluorescence, due to cy3-conjugated secondary antibody, clearly stained the nucleoli within the nucleus. Green fluorescence, due to expression of the transfected fusion construct, exhibited a strict colocalization in heat-shocked cells providing evidence for the nucleolus being the compartment for Mdg1 accumulation during stress (Fig. 7). We then studied whether nucleolar-associated Mdg1 protein could be reexported into the cytosol under recovery conditions. To test this, heat-shocked transfected HeLa cells were allowed to recover at 37 degrees C for 1 and 3 h. The stress-induced translocation of Mdg1 into the nucleoli proved to be reversible within 3 h (Fig. 8), indicating that the localization and transport of Mdg1 is actively controlled by the cell. Furthermore, we compared the cellular localization"; prov:wasQuotedFrom pubmed:11525638 . sub:_5 rdfs:label "Selventa" . sub:assertion prov:hadPrimarySource pubmed:11525638; prov:wasDerivedFrom beldoc:, sub:_4 . } sub:pubinfo { this: dcterms:created "2014-07-03T14:31:43.338+02:00"^^xsd:dateTime; pav:createdBy orcid:0000-0001-6818-334X, orcid:0000-0002-1267-0234 . }