. . . . . . . . . . . . . . . "bp(GOBP:\"response to heat\") -> tloc(p(HGNC:DNAJB9),GOCCID:0005737,GOCCID:0005730)" . "Approximately 61,000 statements." . "Copyright (c) 2011-2012, Selventa. All rights reserved." . "BEL Framework Large Corpus Document" . . "20131211" . "To evaluate whether Mdg1 actually accumulated within the nucleoli, HeLa cells were transfected with pEGFP-Mdg1, heat shocked, and immunostained with a nucleolus-specific antibody. Red-fluorescence, due to cy3-conjugated secondary antibody, clearly stained the nucleoli within the nucleus. Green fluorescence, due to expression of the transfected fusion construct, exhibited a strict colocalization in heat-shocked cells providing evidence for the nucleolus being the compartment for Mdg1 accumulation during stress (Fig. 7). We then studied whether nucleolar-associated Mdg1 protein could be reexported into the cytosol under recovery conditions. To test this, heat-shocked transfected HeLa cells were allowed to recover at 37 degrees C for 1 and 3 h. The stress-induced translocation of Mdg1 into the nucleoli proved to be reversible within 3 h (Fig. 8), indicating that the localization and transport of Mdg1 is actively controlled by the cell. Furthermore, we compared the cellular localization" . . "Selventa" . . . . "2014-07-03T14:31:43.338+02:00"^^ . . .