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@prefix sub: <https://w3id.org/np/RAMVUimnATifJYJw0VP2zAesw4OK_Gw30p9NgcEU4oVW8/> .
@prefix schema: <http://schema.org/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix edam: <http://edamontology.org/> .
@prefix pav: <http://purl.org/pav/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix orcid: <https://orcid.org/> .
@prefix dct: <http://purl.org/dc/terms/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix bibo: <http://purl.org/ontology/bibo/> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix foaf: <http://xmlns.com/foaf/0.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubinfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.org/coloc-seq/method> edam:has_function edam:operation_3680;
    schema:applicationArea "Extracellular vesicle characterization"@en, "Mitochondrial transcriptomics"@en,
      "Organelle RNA localisation"@en;
    schema:description "Controlled Level of Contamination coupled to deep sequencing - a novel subcellular transcriptomics approach"@en;
    schema:name "CoLoC-seq"@en;
    a edam:data_0957, schema:Thing;
    rdfs:comment "Leverages classical enzymatic kinetics to track depletion dynamics of transcripts using RNase gradients"@en .
  
  this: schema:about <https://doi.org/10.1093/nar/gkac1205>;
    schema:text "CoLoC-seq is a novel subcellular transcriptomics approach that uses enzymatic kinetics to distinguish genuine organellar resident RNAs from surface-attached contaminants."@en;
    a <https://w3id.org/np/RAMVUimnATifJYJw0VP2zAesw4OK_Gw30p9NgcEU4oVW8/o/ntemplate/AIDA-Sentence>;
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}

sub:provenance {
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    schema:datePublished "2023"^^xsd:gYear;
    schema:isPartOf sub:__1;
    schema:name "CoLoC-seq probes the global topology of organelle transcriptomes"@en;
    a bibo:AcademicArticle, schema:ScholarlyArticle .
  
  sub:__1 schema:issn "0305-1048";
    schema:name "Nucleic Acids Research"@en;
    a schema:Periodical .
  
  sub:assertion prov:wasAttributedTo orcid:0000-0001-8763-6703, orcid:0000-0002-1481-2996,
      orcid:0000-0002-9142-7979;
    prov:wasDerivedFrom <https://doi.org/10.1093/nar/gkac1205> .
}

sub:pubinfo {
  orcid:0009-0005-7752-8203 foaf:name "Tiado Kollender" .
  
  this: dct:created "2026-01-11T00:00:00Z"^^xsd:dateTime;
    dct:license <https://creativecommons.org/publicdomain/zero/1.0/>;
    npx:signedBy orcid:0009-0005-7752-8203;
    npx:wasCreatedFromPubinfoTemplate <https://w3id.org/np/RA0J4vUn_dekg-U1kK3AOEt02p9mT2WO03uGxLDec1jLw>,
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    pav:createdBy orcid:0009-0005-7752-8203;
    a npx:ExampleNanopub;
    rdfs:comment "AIDA-based assertion defining CoLoC-seq as a novel subcellular transcriptomics approach"@en;
    rdfs:label "Nanopublication: CoLoC-seq core method definition"@en;
    prov:wasAttributedTo orcid:0009-0005-7752-8203 .
  
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