@prefix obo: .
@prefix dce: .
@prefix pplan: .
@prefix this: .
@prefix sub: .
@prefix bs: .
@prefix np: .
@prefix pav: .
@prefix authority: .
@prefix npx: .
@prefix xsd: .
@prefix rdfs: .
@prefix prov: .
sub:Head {
this: np:hasAssertion sub:assertion;
np:hasProvenance sub:provenance;
np:hasPublicationInfo sub:pubInfo;
a np:Nanopublication .
}
sub:assertion {
obo:OBI_0000918 rdfs:label "DMEM" .
sub:_1 rdfs:label "cells" .
sub:step dce:description """Wash cells (centrifuge at 100 x g for 10 min) with 10 ml of sterile DMEM (without FBS) twice.
Note: Cold DMEM is not used routinely for washing lymphocytes from culture cavities while setting up cultures. Rather when monocytes bound tightly to plastic cavities are needed to be harvested pre-chilled DMEM can be used.""";
a pplan:Step;
bs:bioSampleUsed sub:_1;
bs:reagentUsed obo:OBI_0000918 .
}
sub:provenance {
sub:assertion pav:createdWith ;
prov:generatedAtTime "2021-02-14T05:27:45.411427"^^xsd:dateTime;
prov:wasAttributedTo .
}
sub:pubInfo {
sub:assertion prov:wasDerivedFrom authority:0GCTfm6sEeuTj4ssi2Skaw, authority:5bafwG6sEeuROrODpsG-8g,
authority:A5OGZm6tEeuYVk-uglPVIA .
sub:sig npx:hasAlgorithm "RSA";
npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
npx:hasSignature "ba6UiZ8V8p6gOUif5M2DANaOaIcViKw+nkGyxzsJ6OJK3vZnWIAYKjHN4RsJXjzKzMuX+mqaraJnAS3EeZ9b6eXUuvLKfVOXWc0wowgXbhkXegvMbjgDhBQrvf3qbqT1YpPTasRgEB/+GMXhgSmANANz+vVbyH4wIXNDN9DdBMo=";
npx:hasSignatureTarget this: .
this: npx:introduces sub:step;
prov:generatedAtTime "2021-02-14T05:27:45.411427"^^xsd:dateTime;
prov:wasAttributedTo .
}