sub:provenance { beldoc:dce:description "Approximately 61,000 statements." ; dce:rights "Copyright (c) 2011-2012, Selventa. All rights reserved." ; dce:title "BEL Framework Large Corpus Document" ; pav:authoredBysub:_5 ; pav:version "20131211" . sub:_4prov:value "In order to determine the functionality of the TNF-a released in cell supernatants in terms of NF-kB inducibility, we treated ACH-2 cells with equivalent TNF-a concentrations to those released in supernatants after treatment with H2O2 or HOCl, i.e. 100 and 33 pg/ml respectively (or 16.6 and 5 units/ml). Nuclear extracts were prepared 30, 60 and 120 min after induction and analysed by EMSA. As shown in Figure 7(A), a rather important specific NF-kB induction was detectable in the nucleus of ACH-2 cells treated under these conditions. " ; prov:wasQuotedFrompubmed:9032466 . sub:_5rdfs:label "Selventa" . sub:assertionprov:hadPrimarySourcepubmed:9032466 ; prov:wasDerivedFrombeldoc: , sub:_4 . }