@prefix this: <https://w3id.org/np/RAJdsIijwbwE_9GRnUpAgJ0y_E829QmbS6B57uTJDPODI> .
@prefix sub: <https://w3id.org/np/RAJdsIijwbwE_9GRnUpAgJ0y_E829QmbS6B57uTJDPODI/> .
@prefix schema: <http://schema.org/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix edam: <http://edamontology.org/> .
@prefix pav: <http://purl.org/pav/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix orcid: <https://orcid.org/> .
@prefix dct: <http://purl.org/dc/terms/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix sio: <http://semanticscience.org/resource/> .
@prefix bibo: <http://purl.org/ontology/bibo/> .
@prefix prov: <http://www.w3.org/ns/prov#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubinfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:MLVAMethod edam:has_format edam:format_1929;
    edam:has_input edam:data_2044;
    edam:has_output edam:data_2048;
    schema:about <http://purl.obolibrary.org/obo/NCBITaxon_777>;
    schema:alternateName "MLVA"@en;
    schema:citation "Frangoulidis et al."@en;
    schema:description "High-resolution strain discrimination and comparison method based on tandem repeat analysis of 14 loci for Coxiella burnetii genotyping"@en;
    schema:isPartOf <https://coxbase.q-gaps.de>, <https://w3id.org/np/RAnh-o0GrvBfvoyiruq9UnbZOaNcHbue5O-FU6300R_Jg>;
    schema:name "MLVA - Multiple-Locus Variable-Number Tandem-Repeat Analysis"@en;
    schema:potentialAction sub:__1;
    sio:SIO_000216 "14"^^xsd:integer;
    sio:SIO_000229 "high"@en;
    a edam:operation_3196, schema:DefinedTerm;
    rdfs:comment "Calculation formula: RN = (AL - FL) / RS where RN=repeat number, AL=amplicon length, FL=flanking length, RS=repeat size"@en .
  
  sub:__1 schema:instrument sub:__2;
    schema:name "e-PCR amplicon detection"@en;
    a schema:Action .
  
  sub:__2 schema:description "Tool for in silico extraction of MLVA amplicon lengths"@en;
    schema:name "e-PCR tool"@en;
    a schema:SoftwareApplication .
}

sub:provenance {
  <https://doi.org/10.1128/mSystems.00403-21> bibo:doi "10.1128/mSystems.00403-21";
    schema:datePublished "2021"^^xsd:gYear;
    schema:name "CoxBase: an Online Platform for Epidemiological Surveillance, Visualization, Analysis, and Typing of Coxiella burnetii Genomic Sequences"@en;
    a bibo:AcademicArticle, schema:ScholarlyArticle .
  
  sub:assertion prov:generatedAtTime "2026-01-10T23:00:00Z"^^xsd:dateTime;
    prov:wasAttributedTo orcid:0000-0002-1481-2996;
    prov:wasDerivedFrom <https://doi.org/10.1128/mSystems.00403-21> .
}

sub:pubinfo {
  this: dct:created "2026-01-10T23:00:00Z"^^xsd:dateTime;
    dct:creator orcid:0009-0005-7752-8203;
    dct:license <https://creativecommons.org/publicdomain/zero/1.0/>;
    npx:introduces sub:MLVAMethod;
    pav:authoredBy orcid:0009-0005-7752-8203;
    rdfs:comment "This nanopublication describes the MLVA (Multiple-Locus Variable-Number Tandem-Repeat Analysis) genotyping method as implemented in CoxBase for high-resolution strain discrimination of Coxiella burnetii based on 14 tandem repeat loci"@en;
    rdfs:label "MLVA genotyping method for Coxiella burnetii"@en;
    prov:wasAttributedTo orcid:0009-0005-7752-8203 .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIIBIjANBgkqhkiG9w0BAQEFAAOCAQ8AMIIBCgKCAQEAoSR/rqhnkTPQO8u4l2JQAemJo5cN83rT54nA5NXHUqaeu26bEjT/rsX1PCVBhhJ3cV2uu4wjI0NfcVaKPKmODSf6p579tdOykOnjbWLbxxPMe5DC5eV1ThMHsFsNU/6vCYIfS00E7wre7fnFYz7FAG7v1MZEyWeSXsHgk9jHoqQQDPqup5PHReKp1dd6J0Nht/OfSYnUancKAgIFyPhkTlThsLK5xSNyNEgiTR7pwkP5pGq2AnD6bPw4G3kiPnIpIGZNsaeIOvhn64gwbrtoJVdoun/namw6FQ7StEC5km/0evaBZc4qIextwO/s32Wru77ukblJ0Xi/YzbC0iLuIQIDAQAB";
    npx:hasSignature "ejsto3CE3+YKnyxcYWu6aONTqAdd4PctMYAyJluVj7C5opyeHwis3oJtR8sZeRAICFnaPJ+XSgNLjixasqCvWYBnLSgorP41jKPx1tRwJaqUZNMmu1OoC/hQmwWe3nONMMzja4yef37RvUSCyZL+FsxCfAfk22UGR0M4TwqWEgImsym0+uJhJ/7Dmd12Utl8AnxdA2sdaG7hay+2ivLs/d5s+hP3JtI5bOcd5D58kzMzc9MVwntW8Sfuk7OJj2i2s+k0yOqQj54JE/TTwUIGkWVq5qxitMveum/kQILMB3exDws+N/g3QmMQUeNqaCwryOYVkCbN9kTRT6cHXY6HDg==";
    npx:hasSignatureTarget this: .
}