http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw#Head http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw#assertion http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw#provenance http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw#pubinfo http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw#assertion http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw#_1 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10090 http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://amigo.geneontology.org/amigo/term/GO:0014734 http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/increases http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://amigo.geneontology.org/amigo/term/GO:0007520 http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw#assertion http://www.w3.org/2000/01/rdf-schema#label bp(GOBP:"myoblast fusion") -> bp(GOBP:"skeletal muscle hypertrophy") http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw#provenance http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw#_3 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw#_2 http://www.w3.org/ns/prov#value A previous in vitro study demonstrated that myoblasts adhere to a peptide corresponding to the cell-binding carboxyl-terminal end of TSP-4 (Adams and Lawler, 1994). Accordingly, to determine whether TSP-4 can promote myogenic differentiation we carried out coculture experiments with primary myoblasts from newborn mice and TSP-4-expressing COS cells (COS-TSP- 4; Arber and Caroni, 1995). The latter secrete substantial amounts of TSP-4, which was shown in a previous study to efficiently promote neurite outgrowth (Arber and Caroni, 1995). COS-TSP-4 or naive COS cells were plated at low density on laminin-coated culture dishes, and hind limb muscle-derived cells were added 4-5 h later. After 2 d in growth-promoting medium the cultures were switched to differentiation medium, and myotube formation was scored 1 and 2 d later. As shown in Fig. 10, under these experimental conditions, myogenic differentiation and myotube formation were markedly potentiated in the presence of the COS-TSP-4 cells. Muscle-derived cells, which were noticably smaller and thus easily distinguishable from cocultured COS cells, could be detected in comparable amounts in the two types of cultures. However, in the absence of TSP-4 these cells only expressed low levels of muscle-specifi alpha-actinin and only rarely fused to myotubes (Fig. 10). http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw#_2 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/9382869 http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw#_3 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/9382869 http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw#_2 http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw#pubinfo http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw http://purl.org/dc/terms/created 2014-07-03T14:34:02.016+02:00 http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAIcGVLAiB7hBnVNrwOHB7g2vf2bFJqWNKm__nOhgFoIw http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234